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Chinese Journal of Biological Control ›› 2020, Vol. 36 ›› Issue (6): 938-945.DOI: 10.16409/j.cnki.2095-039x.2020.06.024

• RESEARCH REPORTS • Previous Articles    

Effect of High Temperature Film Mulching on Soil Microbial Functional Diversity by High Throughput Sequencing

SONG Jian1, ZHANG Haijian1, LIU Li2, DU Lixin3, LIU Jianhu1, CAO Weiping1   

  1. 1. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding 071000, China;
    2. Hebei Provincial General Station of Plant Protection and Quarantine, Shijiazhuang 050000, China;
    3. College of Life Sciences, Langfang Normal University, Langfang 065000, China
  • Received:2019-12-20 Published:2021-01-09

Abstract: The aim of this study was to clear the effect of high temperature film mulching technology on soil microbial community in the rhizosphere of Chinese chive. The total DNA of Chinese chive rhizosphere soil before and after the high temperature film mulching were extracted, the 16Sr DNA of bacteria and ITS genes of fungal in the soil were sequenced by Illumina Hiseq 2500 high throughput sequencing, and the microbial community structure characteristics of the rhizosphere soil were analyzed. The results showed that the bacteria OTUs and the fungi OTUs before and after the high temperature film mulching were 1849 and 1819, 148 and 151, respectively, and, and there was no significant difference. According to the classification of species before and after the high temperature film mulching, the species of bacteria belonged to 24 phyla, 53 classes, 112 orders, 218 families and 322 genera. The dominant groups were Proteobacteria, Firmicutes and Actinobacteria, and their relative abundances were 43.79%, 21.03%, 21.70% and 42.36%, 23.92%, 17.55%, respectively. Fungal species belonged to 4 phyla, 10 classes, 21 orders, 27 families and 34 genera. The dominant species were Ascomycota and Basidiomycota, and their relative abundances were 62.48%, 15.28% and 51.13%, 40.95%, respectively. The results indicated that the diversity and richness of bacterial community in soil were higher than those of fungi. There was no significant change in total microbial biomass before and after mulching. There was no significant difference in diversity index between bacteria and fungi, but significant difference in structure composition.

Key words: high temperature film mulching, high throughput sequencing, Bradysia odoriphaga, soil microbial functional diversity

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