Effect of Bacillus thuringiensis JQD117 Strain on Intestinal Protease Activity of Larvae of Bradysia odoriphaga

doi:10.16409/j.cnki.2095-039x.2020.04.016

Abstract

Abstract: Effect of Bacillus thuringiensis JQD117 strain on activities of protease and detoxification enzymes of Bradysia odoriphaga larvae, including trypsin, chymotrypsin, acetylcholinesterase (AchE), glutathione-S-transferase (GST), and carboxylesterase (CarE), were determined. The LC₅₀ of JQD117 strain to B. odoriphaga 3^rd instar larvae at 72 h post treatment was determined to be 2.8070×10⁷cfu/mL by laboratory bioactivity assay. Then 10×LC₅₀, 1×LC₅₀ and 0.1×LC₅₀ concentrations of JQD117 were used to feed and infect the B. odoriphaga 3^rd instar larvae. Activities of the five enzymes were measured to the samples regularly collected from the population. The results show that, when the B. odoriphaga 3^rd instar larvae were exposed to high concentrations (1×LC₅₀ and 10×LC₅₀) of JQD117, enzyme activities varied relatively widely. Among them, when the B. odoriphaga 3^rd instar larvae were infected with 1×LC₅₀ and 10×LC₅₀ concentrations of JQD117, trypsin and AchE activities increased significantly at 24-36 h and 6-60 h compared with the control group, while chymotrypsin and CarE activities were significantly inhibited at 6-60 h compared with the control group. When the B. odoriphaga 3^rd instar larvae were infected with 0.1×LC₅₀ concentrations of JQD117, activities of trypsin and AchE were significantly higher than that of the control group only at 36 h and at 12-48 h, respectively; CarE activity was significantly inhibited only at 6 h compared with that of the control group while no significant change in the activity of chymotrypsin was observed. There was no significant change in GST activity at the three infection concentrations compared with the control. Therefore, infection of JQD117 influences on protease and detoxification enzyme activity in B. odoriphaga larvae in a dose-dependent manner. The results provide a theoretical guidance for the application and development of Bt for control of B. odoriphaga.

Key words: Bacillus thuringiensis, Bradysia odoriphaga, protease, detoxifying enzymes

CLC Number:

S476.1

SONG Jian, CAO Weiping, GUO Qinggang, WANG Meng, LI Shezeng, DU Lixin. Effect of Bacillus thuringiensis JQD117 Strain on Intestinal Protease Activity of Larvae of Bradysia odoriphaga[J]. Chinese Journal Of Biological Control, 2020, 36(4): 558-563.

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