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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (3): 547-554.DOI: 10.16409/j.cnki.2095-039x.2021.02.018

• RESEARCH REPORTS • Previous Articles    

Characteristic Analysis and Fumigation Fungistasis Observation of Volatile Organic Compounds from Aroma-producing Fungus GS-1 Strain

WANG Chunsheng1,2, ZHI Yanan1,2, CHEN Lijun1,2, LIU Ming1, HOU Mengyuan1   

  1. 1. Agriculture College, Xinyang Agriculture and Forestry University, Xinyang 464000, China;
    2. Henan Provincial South Henan Crop Pest Green Prevention and Control Academician Workstation, Xinyang 464000, China
  • Received:2020-08-17 Published:2021-06-16

Abstract: The aroma-producing fungus GS−1 strain was isolated from the dead branches of Broussonetia papyrifera, and identified as Nectria cinnabarina. The strain produced rich aroma and its volatile organic compounds (VOCs) had obvious fumigation antifungal activity on many important plant pathogens. In order to determine the fumigation antifungal mechanism, chemical components and dynamic release regularity of the VOCs from GS−1 strain, the fumigation antifungal activity on mycelium and cell structure of Botrytis cinerea were observed by scanning and transmission electron microscopy. The VOCs of GS−1 strain were extracted by HS−SPME. The analysis of dynamic release regularity and identification of the VOCs were carry out by GC−MS. The results showed that the hyphae of B. cinerea was shrunk, deformed and top pointed after treated with VOCs of GS−1 strain. Meanwhile, the cytoplasmic content decreased and the cavities increased. With the passage of cultivation time, the VOCs of GS−1 strain increased firstly, then decreased slightly, tended to be stable at last. It reached the maximum value on the 12th day. Thirty-four chemical components were isolated from the VOCs of GS−1 strain, and 18 of them were identified. The main components were 5−Azulenemethanol,1,2,3,3a,4,5,6,7− octahydro−.alpha.,.alpha.,3,8−tetramethyl−,[3S−(3.alpha.,3a.beta.,5.alpha.)]−, epicubenol and α−patchoulene, respectively accounting for 34.22%, 10.70% and 5.60% in the total VOCs. These results provided a theoretical basis for the rational development and efficient utilization of the VOCs from GS−1 strain in plant disease control.

Key words: VOCs, aroma-producing fungus, antifungal mechanism, GC-MS

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