Analysis of Volatile Compounds from Bacillus subtilis Y13 and Its Antimicrobial Activity

doi:10.16409/j.cnki.2095-039x.2019.04.017

Abstract

Abstract: Bacillus subtilis Y13 is a high-efficiency and broad-spectrum biocontrol strain. It has excellent biocontrol effect on the main diseases of Camellia oleifera. Experiments showed that the volatile substances produced by fermentation had a good inhibitory effect on anthracnose of Camellia oleifera. Volatile substances produced by strain Y13 were analyzed qualitatively and quantitatively, and their bacteriostatic activities were determined, which provided a theoretical basis for pollution-free control of Camellia oleifera diseases and pesticide development. Headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) were used to collect and analyze the volatile components of Y13, purchasing pure volatile substances, and measuring its antifungus activity. The antifungus rate of Y13 volatiles to 10 plant pathogenic fungi was 19.2%-83.9%, among which volatile substances had the strongest antifungus activity against C. fructicola, and the inhibition rate reached 83.9%. The antifungus activity of 10 volatile compounds against C. fructicola was detected. Except octadecane, the other 9 volatile compounds had different degrees of antifungus activity. The four highly effective antifungus volatile compounds were selected. The antifungus activity of pure products against 7 common Camellia oleifera fungi showed that the four volatile compounds (10 μL/plate) had good antifungus activity and antifungus activity against 7 common Camellia oleifera fungi. The antifungus activity were ranked as Nonanal>Benzaldehyde>3-methyl- 4-phenylpyrazole>Benzothiazole. Y13 volatile substances have a good inhibitory effect on the pathogens of Camellia oleifera, and have certain research significance and good development value.

Key words: Bacillus subtilis, volatile compounds, GC-MS, inhibition activity

CLC Number:

S476

FENG Fushan, LIU Junang, HU Liancheng, WEN Ruizhi, ZHOU Guoying. Analysis of Volatile Compounds from Bacillus subtilis Y13 and Its Antimicrobial Activity[J]. Chinese Journal Of Biological Control, 2019, 35(4): 597-604.

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