Welcome to Chinese Journal of Biological Control,Today is

Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (4): 575-580.DOI: 10.16409/j.cnki.2095-039x.2020.04.018

• RESEARCH REPORTS • Previous Articles     Next Articles

Antifungal Activity of Volatile Organic Compounds from Bacillus amyloliquefaciens XJ5 and Control Effect against Monilinia fructigena

GUO Jiexin, ZHANG Yuming, ZHU Honglei, REN Lu, ZHANG Baojun   

  1. College of Agronomy, Shanxi Agricultural University, Taigu 030801, China
  • Received:2020-02-05 Online:2020-08-08 Published:2020-08-07

Abstract: Volatile organic compounds (VOCs) of Bacillus amyloliquefaciens XJ5 have good antifungal activity against various pathogenic fungi such as Monilinia fructigena. The VOCs produced by strain XJ5 were detected by SPME-GC-MS, their antifungal activities were determined by two-sealed-base-plates method, and the control effects against M. fructigena were evaluated in vitro. The results indicated that inhibition rate of XJ5 VOCs against nine pathogenic fungi were ranged between 47.9% and 84.8%, and the highest inhibition rate against M. fructigena was 84.8%. SPME-GC-MS analysis and antifungal activity determination showed that the major VOCs produced by strain XJ5 were Dodecanal, and the major VOCs with antifungal activity were 2-Nonanol, 2-Ethyl-1-hexanol and 2-Undecanol. The EC50 value of 2-Nonanol inhibiting M. fructigena was 3.43 μg/mL. In vitro fumigation with XJ5 bacteria suspension and 2-Nonanol can effectively inhibited the mycelial growth of M. fructigena and the lesion development. The fumigation control efficacies of XJ5 bacteria suspension and 2-Nonanol against M. fructigena were 14.4%~71.2% and 66.4%~97.6%, respectively, significantly different from those of the control.

Key words: Bacillus amyloliquefaciens, volatile organic compounds, SPME-GC-MS, antifungal activity, control effectin in vitro

CLC Number: