Gut Microbiota in the Harlequin Ladybird Beetle Harmonia axyridis Feeding on the Cotton Mealybug Phenacoccus solenopsis

doi:10.16409/j.cnki.2095-039x.2021.07.022

Abstract

Abstract: It remains largely unknown of gut bacterial community of predatory insects in response to non-native food prey.This study examined gut microbiota composition of a generalist predator Harmonia axyridis (Pallas) fed with either non-native mealybugs Phenacoccus solenopsis(Tinsley)(referred to as MFG) or native aphids Megoura japonica (Matsumura)(as AFG) from neonates to adults. The guts of 20-day old predator adults were individually sequenced on theV3-V4 region of the 16S rDNA using the high-throughput sequencing technique to determine the bacterial community.The number of OTUs was nearly 2.3 times higher for MFG than for AFG (297).Among the total 16 phyla uncovered,7 were unique to MFG but none was unique to AFG.Among the total 169 genera detected,210 were unique to MFG while only 35 were unique to AFG.Species diversity indices in 14 genera differed statistically between MFG and AFG,being higher for MFG than for AFG.The function prediction of the gut microbiota indicated that MFG differed from AFG in function abundances though they shared similar metabolic pathways.Our results suggest that H.axyridis adults harbor gut microbiota with higher richness and diversity when feeding on non-native mealybugs than on native aphids.

Key words: Coccinellidae, gut microbiome, biodiversity, invasion biology, prey-predator interaction

CLC Number:

S476.2

GAO Ping, WANG Yansong, GUO Longxiu, LI Baoping. Gut Microbiota in the Harlequin Ladybird Beetle Harmonia axyridis Feeding on the Cotton Mealybug Phenacoccus solenopsis[J]. Chinese Journal of Biological Control, 2022, 38(4): 967-974.

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