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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (2): 245-253.DOI: 10.16409/j.cnki.2095-039x.2022.03.031

• RESEARCH REPORTS •     Next Articles

Reproductive Phenotype of Two Wolbachia-infected Thelytokous Line of Trichogramma dendrolimi Matsumura

Lü Lin1, LI Yuanyuan1, HUO Liangxiao1, LIANG Hongyu1, ZHANG Huanhuan2, ZHOU Jincheng1,3, ZHANG Lisheng3, DONG Hui1   

  1. 1. College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China;
    2. Institute of Vegetable, Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa 850032, China;
    3. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2022-03-08 Published:2023-04-13

Abstract: The effects of temperature, genetic background, and age on the reproductive phenotype and Wolbachia titer of Wolbachia-infected thelytokous line of Trichogramma dendrolimi Matsumura were investigated by analyzing the parameters of reproductive phenotype (male proportion and intersex proportion), fitness (numbers of parasitized host eggs and emergence proportion), and Wolbachia titer as influenced by temperature (22 ℃ and 29 ℃), female age, and genetic background (ZW line and XF line). The results showed that the male proportion and intersex proportion at 29 ℃ were significantly higher than those at 22 ℃. However, the Wolbachia titer and the number of daily parasitized host eggs deposited by maternal females at 29 ℃ were significantly lower than those at 22 ℃. The male proportion of ZW line was significantly higher than that of XF line, but the number of daily parasitized host eggs of ZW line was significantly lower than that of XF line. The male proportion and intersex proportion of offspring increased significantly with the age of maternal females, but the number of daily parasitized host eggs and emergence proportion of offspring decreased significantly with the age of maternal females. The results highlight the significant effects of temperature and age on the reproductive phenotype of Wolbachia-infected thelytokous line of Trichogramma dendrolimi Matsumura with different genetic background. The research is expected to help the improvement of the mass-rearing procedure and field application of thelytokous Trichogramma in biological control programs.

Key words: Wolbachia, Trichogramma dendrolimi, thelytokous parthenogernesis, gentic background

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