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Chinese Journal of Biological Control ›› 2022, Vol. 38 ›› Issue (4): 821-830.DOI: 10.16409/j.cnki.2095-039x.2022.04.008

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Biocontrol Activities and Metabolome Analysis of Microsclerotia Fermentation Filtrate of Purpureocillium lilacinum

FAN Lele, GUO Yan, ZHAO Xue, SUN Manhong, LI Shidong   

  1. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2022-06-26 Published:2022-08-11

Abstract: Purpureocillium lilacinum is an important biocontrol fungus,which can produce a kind of resistant structure -microsclerotia under given culture conditions.In this study,the control efficiencies of microsclerotia fermentation filtrate of P.lilacinum against plant fungal pathogens and root-knot nematode were investigated by antagonism in the lab and pot experiment in greenhouse.The results showed that the filtrate of microsclerotia had strong inhibition to nematodes and pathogenic fungi.The growth inhibition rate to Sclerotinia sclerotiorum reached 70.5%,and the yield of sclerotia significantly decreased.The nematicidal activity of the filtrate to the juveniles of root-knot nematode was over 50% when the filtrate was diluted 10 times.In greenhouse,when 10 mL/seedling was used,the control efficiency against root-knot nematode in cucumber reached 44.9%,and the height and stem diameter of the seedlings increased by 21.6% and 38.4%,respectively.Additionally,the metabolome of P.lilacinum microsclerotia fermentation was constructed basing on LC-MS analysis,and the results showed that the relative contents of anti-microbial compounds,such as erucic acid,piperol and pantothenic acid,and the metabolites with nematicidal activities such as butyric acid and imidazolacetic acid,increased significantly.This study lays a foundation to understand the biocontrol mechanisms of P.lilacinum and facilitates the development of fungal agents composed of microsclerotia.

Key words: Purpureocillium lilacinum, microsclerotia, root-not nematode egg, fermentation filtrate, metabolome

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