Control of Root-Knot Nematode in Cucumber with Purpureocillium lilacinum Microsclerotia and Effects of Soil Factors on Fungal Colonization

doi:10.16409/j.cnki.2095-039x.2022.04.007

Abstract

Abstract: Microsclerotia (MS) is a kind of resistant structure produced by mycelia specializing of some filamentous fungi.In previous studies,we found that Purpureocillium lilacinum could produce microsclerotia under some given culture conditions,which were resistant to adverse environmental stresses.Here,control efficiency of P.lilacinum microsclerotia against root-knot nematode in cucumber and the effects of soil conditions on fungal colonization were investigated in the lab and greenhouse.The results showed that the parasitism rates of the microsclerotia increased with the increase of inoculation concentration.When 300 MS/mL was added,the parasitism rate to nematode eggs was over 50%.The control efficiency of microsclerotia on nematodes in cucumber reached to 44.7% when 300 MS/100 g soil was applied during transplanting.Additionally,it was enhanced by 36.0% with the treatment of microsclerotia in seedling substrates ahead.Soil conditions,such as moisture,temperature and pH had remarkable influence on colonization of P.lilacinum microsclerotia (P<0.05).When soil water contents were 10%-15%,the population densities of the biocontrol fungus increased by 6-9 times compared to those under much high or low moisture.The above results provide theoretical guidance for the development and application of P.lilacinum agents composed of the resistant structure of microsclerotia.

Key words: Purpureocillium lilacinum, microsclerotia, root-not nematode egg, biological control

CLC Number:

S476

YUAN Menglei, FAN Lele, ZHAO Xue, ZHONG Zengming, SUN Manhong, LI Shidong. Control of Root-Knot Nematode in Cucumber with Purpureocillium lilacinum Microsclerotia and Effects of Soil Factors on Fungal Colonization[J]. Chinese Journal of Biological Control, 2022, 38(4): 831-839.

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