Isolation, Identification and the Virulence of Metarhizium rileyi from Adult of Spodoptera frugiperda (J. E. Smith)

doi:10.16409/j.cnki.2095-039x.2022.11.029

Abstract

Abstract: A strain of entomopathogenic fungi was isolated from the adult of Spodoptera frugiperda. On the base of morphological and molecular analyses, this strain was identified as Metarhizium rileyi and named NrSfadult202104. In this study, the phenotypic characteristics of the strain were evaluated. The maximal growth rate of colony and conidial production were on the SMAY medium containing puparium powder. The optimal temperatures for colony growth and conidial production were 28 ℃ and 20 ℃, respectively. The maximum conidial production was achieved under the 24h-illumination condition. This strain was pathogenic to adults and the 3^rd instar larvae of S. frugiperda, the corrected mortality of infected female and male adults were 61.2% and 58.1%, respectively with the inoculation of conidial concentration of 1.0×10⁸ spores/mL. The mortality of 3^rdinstar larvae increased with the increase of the conidial concentration, the median lethal time (LT₅₀) of the larvae was 5.30 days with the concentration of 1.0×10⁸ spores/mL, and the corrected mortality reached 66.12% on the 8th day. In summary, the strain NrSfadult202104 shows a good potential as a biocontrol agent for S. frugiperda and is worth of further research.

Key words: Spodoptera frugiperda, Metarhizium rileyi, adults

CLC Number:

S476.1

PANG Jixin, XIAO Guanli, DU Guangzu, LI Zhengyue, CHEN Bin, PENG Yuejin. Isolation, Identification and the Virulence of Metarhizium rileyi from Adult of Spodoptera frugiperda (J. E. Smith)[J]. Chinese Journal of Biological Control, 2023, 39(2): 331-339.

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