Screening of a Metarhizium rileyi Strain and Its Virulences to Spodoptera frugiperda (Lepidoptera: Noctuidae)

doi:10.16409/j.cnki.2095-039x.2021.05.015

Abstract

Abstract: It is crucial for green prevention and control of Spodoptera frugiperda to collect and screen biocontrol strains with high virulence against S. frugiperda. In this study, a fungus was isolated from diseased larvae of S. frugiperda collected in Nanning, Guangxi. The species was identified using morphological characterization and ITS-rDNA sequence analysis, and the pathogenicity of the strain to the larvae, prepupa, and pupa of S. frugiperda were determined by immersion method in the laboratory. The pathogen was identified as Metarhizium rileyi, named CDTLJ1, could infect the larvae, prepupa, and pupa of S. frugiperda. The 1.20×10⁷ conidia/mL concentration treatment caused 100%, 100%, 100%, 100%, 98.33%, 28.33%, and 20.00% corrected mortality of 2^nd, 3^rd, 4^th, 5^th and 6^th instar larvae, prepupa, and pupa on the 6^th day post treatment, and resulted in median lethal time (LT50) values of 3.91 d—4.24 d for the 2^nd to 6^th instar larvae. The mortality rate of the 3^rd instar larvae caused by infection of M. rileyi strain CDTLJ1 increased with the rising of concentration of conidial suspension. When the concentration reached 1×10⁸ conidia/mL, LT50 was 4.10 d, the corrected accumulative mortality reached 100% and the median lethal concentration (LC₅₀) was 1.12×10⁴ conidia/mL on the 6^th day post treatment. These results show that the M. rileyi strain CDTLJ1 has a high biocontrol potential for use against S. frugiperda and deserves further research and utilization.

Key words: Spodoptera frugiperda, Metarhizium rileyi, virulences, identification

CLC Number:

S476.1

LONG Xiuzhen, GAO Xuyuan, ZENG Xianru, HE Zhan, WEI Dewei, JIANG Xiaodong, ZENG Tao, YU Yonghao. Screening of a Metarhizium rileyi Strain and Its Virulences to Spodoptera frugiperda (Lepidoptera: Noctuidae)[J]. Chinese Journal of Biological Control, 2021, 37(6): 1111-1119.

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