Rhizosphere Colonization of Plant Growth Promoting Rhizobacteria HQ1-2 and Regulation Effects of Soil Microecology and Control Effects on the Fusarium Wilt

doi:10.16409/j.cnki.2095-039x.2023.02.034

Abstract

Abstract: In order to study the colonization ability of rhizobacteria HQ1-2 and effects its on rhizosphere microecology and Fusarium wilt of cucumber, Its colonization density of HQ1-2 were detected by dilution plating, the antifungal activity of HQ1-2 against Fusarium oxysporum were detected by in vitro experiments, the control effect of HQ1-2 against Fusarium wilt of cucumber and on rhizosphere soil microecology by pot experiments. The results showed that, HQ1-2 could colonize stable in cucumber roots and rhizosphere soil, 12 days after treatment, HQ1-2 could maintain a density of 10⁴ and 10⁶cfu/g, respectively in cucumber root and its rhizosphere soil. The treatment with HQ1-2 increse the bacteria and actinomycetes, while decresing the fungi quantity in the rhizosphere soil, remarkably improve urease, phosphatase, sucrase and catalase activities in cucumber rhizosphere soil, increase the available NPK contents. HQ1-2 was able to inhibit the mycelia growth, conidia formation and conidia germination of Fusarium oxysporum significantly in the in vitro experiment, HQ1-2 performed the successful potential control against Fusarium wilt of cucumber in the pot experiment, the control efficiencies was 84.62%. Based on the morphological,physiological characteristics and 16S rRNA sequence analysis,the strain HQ1-2 was identified as Paenibacillus polymyxa.

Key words: plant growth-promoting rhizobacteria, rhizosphere colonization, soil microecology, Fusarium wilt of cucumber, Paenibacillus polymyxa

CLC Number:

S476

CHEN Mengduo, HU Chunyan, MA Xiaojing, HE Menghan, SHEN Husheng, WANG Yiru, PIAO Fengzhi, SHEN Shunshan. Rhizosphere Colonization of Plant Growth Promoting Rhizobacteria HQ1-2 and Regulation Effects of Soil Microecology and Control Effects on the Fusarium Wilt[J]. Chinese Journal of Biological Control, 2023, 39(4): 924-932.

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