Effects of Soil Factors on the Colonization of Marine Biocontrol Bacterium Paenibacillus polymyxa L1-9 in Cucumber Root Surface Soil

doi:10.16409/j.cnki.2095-039x.2017.05.014

Abstract

Abstract: In this study, the effects of soil organic matter contents, temperature and soil pH value on colonization of marine biocontrol bacterium of L₁-9 in root surface soil were investigated. 16S rDNA gene fragments of the strain in the root surface soil were quantified using quantitative real-time PCR method. The results showed that soil organic matter content, temperature and pH exhibited significant impacts on colonization of the strain L₁-9 in root surface soil (P<0.01). The propagation of the strain L₁-9 in root surface soil was faster under the temperature of 25℃, which was 5.79×10⁸ copies per gram dry soil, and 4.3 times of that under 15℃. Addition of organic matter (20-40 g/kg) increased the population density of the strain L₁-9. The maximum colonization level of the strain L₁-9 in the root surface soil was achieved in pH 7. This study laid a foundation for the development and application of the marine biocontrol bacterium L₁-9 against soilborne diseases of cucumber.

Key words: Paenibacillus polymyxa, root surface soil, colonization, quantitative real-time PCR

CLC Number:

S476

WANG Shufang, WANG Jingjing, CAO Xuemei, LI Huan, WANG Qi, WANG Junqiang, MA Guizhen, BAO Zenghai. Effects of Soil Factors on the Colonization of Marine Biocontrol Bacterium Paenibacillus polymyxa L₁-9 in Cucumber Root Surface Soil[J]. journal1, 2017, 33(5): 675-680.

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Effects of Soil Factors on the Colonization of Marine Biocontrol Bacterium Paenibacillus polymyxa L₁-9 in Cucumber Root Surface Soil

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