Isolation and Identification of Biocontrol Bacterium ACQ-03 against Sclerotinia sclerotiorum on Oilseed Rape and Its Biological Activity

doi:10.16409/j.cnki.2095-039x.2025.02.053

Abstract

Abstract: Sclerotinia stem rot caused by Sclerotinia sclerotiorum severely impacts the yield and quality of oilseed rape. To explore novel green control strategies, a bacterial strain designated ACQ-03, with a mycelial growth inhibition rate of 66.52% against S.sclerotiorum was isolated from oilseed rape rhizosphere soil using dilution-plating and dual-culture assays. Based on morphological characteristics, physiological and biochemical tests, as well as 16S rRNA and gyrB gene sequence analyses, strain ACQ-03 was identified as Bacillus subtilis. The biological activity study results showed that ACQ-03 effectively inhibited mycelial growth of the pathogen and significantly reduced sclerotial germination. Detached leaf assays demonstrated that its efficacy against oilseed rape stem rot reached 83.6%, while also substantially reducing disease incidence. Further antifungal spectrum tests indicated that ACQ-03 exhibited strong inhibitory effects on 18 common phytopathogenic fungi including Exserohilum turcicum, Coniella diplodiella, and Sclerotinia homoeocarpa. In summary, these results highlight the potential of Bacillus subtilis ACQ-03 as a biocontrol agent for managing Sclerotinia stem rot in oilseed rape and other plant diseases.

Key words: Sclerotinia stem rot, Bacillus subtilis, biological control, identification

CLC Number:

S476

YI Dongyin, YUAN Shankui, BI Yang, Ma Chunying. Isolation and Identification of Biocontrol Bacterium ACQ-03 against Sclerotinia sclerotiorum on Oilseed Rape and Its Biological Activity[J]. Chinese Journal of Biological Control, 2025, 41(4): 939-948.

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