Isolation and Identification of Bacillus amyloliquefaciens Z3 and Control Effect of Its Metabolites on Sugar Beet Damping-off

doi:10.16409/j.cnki.2095-039x.2026.02.040

Abstract

Abstract: Sugar beet is an important sugar crop, and damping-off caused by Rhizoctonia solani poses a serious threat to its yield and quality. To achieve green and efficient control of this disease, a total of 354 bacterial strains were isolated from the leaves, stems, roots and rhizosphere soil of healthy sugar beet seedlings. This strain showed an inhibition rate of 79.83% against R. solani under in vitro conditions, and its protective efficacy against sugar beet damping-off reached 70%. Based on morphological characteristics, physiological and biochemical reactions, 16S rRNA and gyr B gene sequence characteristics, strain Z3 was identified as Bacillus amyloliquefaciens. Greenhouse disease control test demonstrated that the sterile filtrate of strain Z3 had a control efficacy of 85% against sugar beet damping-off. Furthermore, non-targeted metabolomics combined with bioactivity validation revealed that quinaldic acid and menadione produced by strain Z3 significantly suppressed R. solani growth, with IC₅₀ values of 67.19 and 7.55 mmol/L, and control efficacies of 40% and 55% in pot assays, respectively. These findings indicate that B. amyloliquefaciens Z3 and its bioactive metabolites hold considerable application potential for the biocontrol of sugar beet damping-off.

Key words: sugar beet damping-off, Rhizoctonia solani, Bacillus amyloliquefaciens, metabolite, biological control

CLC Number:

S476

ZHANG Ya, WANG Weixiong, SUN Jiameng, WU Di, HU Xiaohang, NIU Ben. Isolation and Identification of Bacillus amyloliquefaciens Z3 and Control Effect of Its Metabolites on Sugar Beet Damping-off[J]. Chinese Journal of Biological Control, 2026, 42(4): 934-944.

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