Research Progress on Biocontrol Bacillus spp. for Plant Disease Management

doi:10.16409/j.cnki.2095-039x.2026.02.032

Abstract

Abstract: Plant diseases pose a serious threat to global agricultural productivity and crop quality. Long-term reliance on chemical pesticides for disease control has triggered various challenges, including increased pathogen resistance, pesticide residues, and environmental pollution. Consequently, environmentally friendly biological control strategies have attracted increasing attention. Bacillus spp. are important plant-beneficial microorganisms and show great potential in the biological control of plant diseases. In recent years, advances in molecular biology and multi-omics technologies have significantly promoted the exploration of Bacillus resources and the elucidation of their multifaceted biocontrol mechanisms, as well as the development and industrial application of Bacillus-based agents. This review systematically summarizes recent advances in biocontrol Bacillus, including resource exploration, mechanisms of action, and application development, and discusses future research priorities and application prospects.

Key words: plant diseases, Bacillus spp., biological control, mechanism, product application

CLC Number:

S476

LIU Ziyi, WANG Zhenshuo, LI Yan, ZENG Qingchao, WANG Qi. Research Progress on Biocontrol Bacillus spp. for Plant Disease Management[J]. Chinese Journal of Biological Control, 2026, 42(2): 317-327.

References

[1] Godfray H C J, Beddington J R, Crute I R, et al. Food security: the challenge of feeding 9 billion people[J]. Science, 2010, 327(5967): 812-818.
[2] Zhang N, Wang Z, Shao J, et al. Biocontrol mechanisms of Bacillus: Improving the efficiency of green agriculture[J]. Microbial Biotechnology, 2023, 16(12): 2250-2263.
[3] Lahlali R, Ezrari S, Radouane N, et al. Biological control of plant pathogens: a global perspective[J]. Microorganisms, 2022, 10(3): 596.
[4] 彭钿钿, 马云龙, 许沛冬, 等. 芽胞杆菌防治植物病害作用机制与应用[J]. 生物技术通报, 2025, 8(41): 42-52.
[5] Earl A M, Losick R, Kolter R. Ecology and genomics of Bacillus subtilis[J]. Trends in Microbiology, 2008, 16(6): 269-275.
[6] Akinsemolu A A, Onyeaka H, Odion S, et al. Exploring Bacillus subtilis: Ecology, biotechnological applications, and future prospects[J]. Journal of Basic Microbiology, 2024, 64(6): 2300614.
[7] Nicholson W L, Munakata N, Horneck G, et al. Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments[J]. Microbiology and Molecular Biology Reviews, 2000, 64(3): 548-572.
[8] 王阶平, 刘波, 刘国红, 等. 芽胞杆菌系统分类研究最新进展[J]. 福建农业学报, 2017, 32(7): 784-800.
[9] Li P, Tedersoo L, Crowther TW, et al. Fossil-fuel-dependent scenarios could lead to a significant decline of global plant-beneficial bacteria abundance in soils by 2100[J]. Nature Food, 2023, 4(11): 996-1006.
[10] Patel S, Gupta R S. A phylogenomic and comparative genomic framework for resolving the polyphyly of the genus Bacillus: Proposal for six new genera of Bacillus species, Peribacillus gen. nov., Cytobacillus gen. nov., Mesobacillus gen. nov., Neobacillus gen. nov., Metabacillus gen. nov. and Alkalihalobacillus gen. nov.[J]. International Journal of Systematic and Evolutionary Microbiology, 2020, 70(1): 406-438.
[11] Zhao M, Liu D, Liang Z, et al. Antagonistic activity of Bacillus subtilis CW14 and its β-glucanase against Aspergillus ochraceus[J]. Food Control, 2022, 131: 108475.
[12] Deng Y, Chen Z, Ruan C, et al. Antifungal activities of Bacillus velezensis FJAT 52631 and its lipopeptides against anthracnose pathogen ‐ Colletotrichum acutatum[J]. Journal of Basic Microbiology, 2023, 63(6): 594-603.
[13] Tran TD, Del Cid C, Hnasko R, et al. Bacillus amyloliquefaciens ALB65 inhibits the growth of Listeria monocytogenes on cantaloupe melons[J]. Applied and Environmental Microbiology, 2020, 87(1): e01926-20.
[14] 邴辉, 王仲康, 杜秉海, 等. 基因组关联分析地衣芽孢杆菌LCDD6对核桃苗的促生作用[J]. 应用与环境生物学报, 2022, 28(3): 588-595.
[15] Hao K, Ullah H, Qin X, et al. Effectiveness of Bacillus pumilus PDSLzg-1, an innovative hydrocarbon-degrading bacterium conferring antifungal and plant growth-promoting function[J]. 3 Biotech, 2019, 9(8): 305.
[16] Ajdig M, Mbarki A, Chouati T, et al. Comprehensive genomic and pan-genomic analysis of the drought-tolerant Bacillus halotolerans strain OM-41 isolated from olive rhizosphere, reveals potential plant growth-promoting and biocontrol traits[J]. World Journal of Microbiology and Biotechnology, 2025, 41(8): 276.
[17] Sorokan A, Gabdrakhmanova V, Kuramshina Z, et al. Plant-associated Bacillus thuringiensis and Bacillus cereus: inside agents for biocontrol and genetic recombination in phytomicrobiome[J]. Plants, 2023, 12(23): 4037.
[18] Sharga B M, Lyon G D. Bacillus subtilis BS 107 as an antagonist of potato blackleg and soft rot bacteria[J]. Canadian Journal of Microbiology, 1998, 44(8): 777-783.
[19] Blake C, Christensen M N, Kovács Á T. Molecular aspects of plant growth promotion and protection by Bacillus subtilis[J]. Molecular Plant-Microbe Interactions^®, 2021, 34(1): 15-25.
[20] Santoyo G, Urtis-Flores C A, Loeza-Lara P D, et al. Rhizosphere colonization determinants by plant growth-promoting rhizobacteria (PGPR)[J]. Biology-Basel, 2021, 10(6): 475.
[21] Grady E N, MacDonald J, Liu L, et al. Current knowledge and perspectives of Paenibacillus: A review[J]. Microbial Cell Factories, 2016, 15(1): 203.
[22] 刘悦, 曾凡松, 龚双军, 等. 解淀粉芽胞杆菌EA19菌株对小麦赤霉病的防治效果[J]. 植物保护学报, 2020, 47(6): 1270-1276.
[23] 蒲欣, 吴茂华, 刘锋, 等. 芽孢杆菌对玉米真菌病害生物防治效果的研究进展[J]. 江苏农业科学, 2024, 52(4): 23-30.
[24] 乔俊卿, 陈志谊, 梁雪杰, 等. 枯草芽孢杆菌Bs916防治番茄青枯病[J]. 中国生物防治学报, 2016, 32(2): 229-234.
[25] 张晓云, 王雪美, 丛蓉, 等. 防治葡萄灰霉病的贝莱斯芽胞杆菌HMB28023筛选及其抑菌物质[J]. 中国生物防治学报, 2025, 41(1): 122-131.
[26] Raymaekers K, Ponet L, Holtappels D, et al. Screening for novel biocontrol agents applicable in plant disease management – A review[J]. Biological Control, 2020, 144: 104240.
[27] Pliego C, Ramos C, de Vicente A, et al. Screening for candidate bacterial biocontrol agents against soilborne fungal plant pathogens[J]. Plant and Soil, 2011, 340(1): 505-520.
[28] 康兴娇, 申红妙, 贾招闪, 等. 葡萄霜霉病生防菌甲基营养型芽胞杆菌T3的鉴定及其防治效果[J]. 中国生物防治学报, 2016, 32: 775-782.
[29] 孙旺旺, 闫丽, 陈昌龙, 等. 生菜软腐和菌核病拮抗菌贝莱斯芽胞杆菌BPC6鉴定与防效[J]. 中国生物防治学报, 2020, 36(2): 231-240.
[30] 陈川雁, 王燕, 喻国辉, 等. 枯草芽胞杆菌R31影响巴西蕉根系活性氧产生及对枯萎病的防治效果[J]. 中国生物防治学报, 2017, 33(2): 226-233.
[31] 何朋杰, 崔文艳, 何鹏飞, 等. 叶面喷施枯草芽胞杆菌XF-1防治大白菜根肿病[J]. 植物保护, 2019, 45(1): 104-108.
[32] 李新宇, 李磊, 石延霞, 等. 黄瓜棒孢叶斑病拮抗细菌的筛选、鉴定及防治效果[J]. 植物保护学报, 2020, 47(3): 620-627.
[33] 孟焕文, 赵远征, 梁东超, 等. 马铃薯黑胫病生防菌株的筛选与鉴定[J]. 中国植保导刊, 2020, 40(8): 24-29.
[34] Ayaz M, Li C-H, Ali Q, et al. Bacterial and fungal biocontrol agents for plant disease protection: journey from lab to field, current status, challenges, and global perspectives[J]. Molecules, 2023, 28(18): 6735.
[35] 黎永坚, 程萍, 喻国辉, 等. 枯草芽孢杆菌R31和 TR21菌株防治香蕉枯萎病田间药效试验[J]. 广东农业科学, 2012, 39(23): 70-72.
[36] 王鹏程, 金光辉, 张春雨, 等. 不同生防菌剂组合及施用方式对马铃薯疮痂病的防治效果及促生作用[J]. 西南农业学报, 2022, 35(4): 797-803.
[37] Elnahal A S M, El-Saadony M T, Saad A M, et al. The use of microbial inoculants for biological control, plant growth promotion, and sustainable agriculture: A review[J]. European Journal of Plant Pathology, 2022, 162(4): 759-792.
[38] Valenzuela Ruiz V, Cervantes Enriquez E P, Vázquez Ramírez M F, et al. A new era in the discovery of biological control bacteria: omics-driven bioprospecting[J]. Soil Systems, 2025, 9(4): 108.
[39] Xia L, Miao Y, Cao A, et al. Biosynthetic gene cluster profiling predicts the positive association between antagonism and phylogeny in Bacillus[J]. Nature Communications, 2022, 13(1): 1023.
[40] Massart S, Martinez-Medina M, Jijakli M H. Biological control in the microbiome era: challenges and opportunities[J]. Biological Control, 2015, 89: 98-108.
[41] Muñoz C Y, Zhou L, Yi Y, et al. Biocontrol properties from phyllospheric bacteria isolated from Solanum lycopersicum and Lactuca sativa and genome mining of antimicrobial gene clusters[J]. BMC Genomics, 2022, 23(1): 152.
[42] Gao M, Xiong C, Gao C, et al. Disease-induced changes in plant microbiome assembly and functional adaptation[J]. Microbiome, 2021, 9(1): 187.
[43] Wang R, Zhang W, He Z, et al. Core microbiota recruited by healthy grapevines enhance resistance against root rot disease[J]. Genome Biology, 2026, 27(1): 13.
[44] Biggs M B, Craig K, Gachango E, et al. Genomics-and machine learning-accelerated discovery of biocontrol bacteria[J]. Phytobiomes Journal, 2021, 5(4): 452-463.
[45] Fira D, Dimkić I, Berić T, et al. Biological control of plant pathogens by Bacillus species[J]. Journal of Biotechnology, 2018, 285: 44-55.
[46] Calvo H, Mendiara I, Arias E, et al. Antifungal activity of the volatile organic compounds produced by Bacillus velezensis strains against postharvest fungal pathogens[J]. Postharvest Biology and Technology, 2020, 166: 111208.
[47] Abriouel H, Franz C M A P, Ben Omar N, et al. Diversity and applications of Bacillus bacteriocins[J]. FEMS Microbiology Reviews, 2011, 35(1): 201-232.
[48] Dimkić I, Janakiev T, Petrović M, et al. Plant-associated Bacillus and Pseudomonas antimicrobial activities in plant disease suppression via biological control mechanisms - A review[J]. Physiological and Molecular Plant Pathology, 2022, 117: 101754.
[49] Scholz R, Vater J, Budiharjo A, et al. Amylocyclicin, a novel circular bacteriocin produced by Bacillus amyloliquefaciens FZB42[J]. Journal of Bacteriology, 2014, 196(10): 1842-1852.
[50] Shelburne CE, An FY, Dholpe V, et al. The spectrum of antimicrobial activity of the bacteriocin subtilosin A[J]. Journal of Antimicrobial Chemotherapy, 2007, 59(2): 297-300.
[51] Ongena M, Jacques P. Bacillus lipopeptides: versatile weapons for plant disease biocontrol[J]. Trends in Microbiology, 2008, 16(3): 115-125.
[52] 马佳, 李颖, 胡栋, 等. 芽胞杆菌生物防治作用机理与应用研究进展[J]. 中国生物防治学报, 2018, 34(4): 639-648.
[53] Bais H P, Fall R, Vivanco J M. Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production[J]. Plant Physiology, 2004, 134(1): 307-319.
[54] Romero D, de Vicente A, Rakotoaly R H, et al. The iturin and fengycin families of lipopeptides are key factors in antagonism of Bacillus subtilis toward Podosphaera fusca[J]. Molecular Plant-Microbe Interactions, 2007, 20(4): 430-440.
[55] Sui X, Han X, Cao J, et al. Biocontrol potential of Bacillus velezensis EM-1 associated with suppressive rhizosphere soil microbes against tobacco bacterial wilt[J]. Frontiers in Microbiology, 2022, 13: 940156.
[56] Zhang L, Sun C. Fengycins, Cyclic lipopeptides from marine Bacillus subtilis strains, kill the plant-pathogenic fungus Magnaporthe grisea by inducing reactive oxygen species production and chromatin condensation[J]. Applied and Environmental Microbiology, 2018, 84(18): e00445-18.
[57] Wu L, Wu H, Chen L, et al. Difficidin and bacilysin from Bacillus amyloliquefaciens FZB42 have antibacterial activity against Xanthomonas oryzae rice pathogens[J]. Scientific Reports, 2015, 5(1): 12975.
[58] Milner J, Raffel S, Lethbridge B, et al. Culture conditions that influence accumulation of zwittermicin A by Bacillus cereus UW85[J]. Applied Microbiology and Biotechnology, 1995, 43(4): 685-691.
[59] Zhang D, Yu S, Yang Y, et al. Antifungal effects of volatiles produced by Bacillus subtilis against Alternaria solani in potato[J]. Frontiers in Microbiology, 2020, 11: 1196.
[60] Kai M, Haustein M, Molina F, et al. Bacterial volatiles and their action potential[J]. Applied Microbiology and Biotechnology, 2009, 81(6): 1001-1012.
[61] Xu W, Yang Q, Yang F, et al. Evaluation and genome analysis of Bacillus subtilis YB-04 as a potential biocontrol agent against Fusarium wilt and growth promotion agent of cucumber[J]. Frontiers in Microbiology, 2022, 13: 885430.
[62] Luo L, Zhao C, Wang E, et al. Bacillus amyloliquefaciens as an excellent agent for biofertilizer and biocontrol in agriculture: An overview for its mechanisms[J]. Microbiological Research, 2022, 259: 127016.
[63] Arnaouteli S, Bamford N C, Stanley-Wall N R, et al. Bacillus subtilis biofilm formation and social interactions[J]. Nature Reviews Microbiology, 2021, 19(9): 600-614.
[64] Zhang Y, Gao X, Wang S, et al. Application of Bacillus velezensis NJAU-Z9 enhanced plant growth associated with efficient rhizospheric colonization monitored by qPCR with primers designed from the whole genome sequence[J]. Current Microbiology, 2018, 75(12): 1574-1583.
[65] Li S, Zhang N, Zhang Z, et al. Antagonist Bacillus subtilis HJ5 controls Verticillium wilt of cotton by root colonization and biofilm formation[J]. Biology and Fertility of Soils, 2013, 49(3): 295-303.
[66] Dimopoulou A, Theologidis I, Benaki D, et al. Direct antibiotic activity of bacillibactin broadens the biocontrol range of Bacillus amyloliquefaciens MBI600[J]. Msphere, 2021, 6(4): 10-1128.
[67] Pirhonen M, Flego D, Heikinheimo R, et al. A small diffusible signal molecule is responsible for the global control of virulence and exoenzyme production in the plant pathogen Erwinia carotovora[J]. EMBO Journal, 1993, 12(6): 2467-2476.
[68] Krzyzanowska DM, Potrykus M, Golanowska M, et al. Rhizosphere bacteria as potential biocontrol agents against soft rot caused by various Pectobacterium and Dickeya spp. strains[J]. Journal of Plant Pathology, 2012, 94(2): 367-378.
[69] Yu Y, Gui Y, Li Z, et al. Induced systemic resistance for improving plant immunity by beneficial microbes[J]. Plants-Basel, 2022, 11(3): 386.
[70] Ongena M, Jourdan E, Adam A, et al. Surfactin and fengycin lipopeptides of Bacillus subtilis as elicitors of induced systemic resistance in plants[J]. Environmental Microbiology, 2007, 9(4): 1084-1090.
[71] Huang C J, Tsay J F, Chang S Y, et al. Dimethyl disulfide is an induced systemic resistance elicitor produced by Bacillus cereus C1L[J]. Pest Management Science, 2012, 68(9): 1306-1310.
[72] Tsotetsi T, Nephali L, Malebe M, et al. Bacillus for plant growth promotion and stress resilience: what have we learned?[J]. Plants-Basel, 2022, 11(19): 2482.
[73] Shao M-W, Chen H-J, Huang A-Q, et al. Modulation of rhizosphere microbiota by Bacillus subtilis R31 enhances long-term suppression of banana Fusarium wilt[J]. iMetaOmics, 2025, 2(2): e70006.
[74] Liu Y, Zhang H, Wang J, et al. Nonpathogenic Pseudomonas syringae derivatives and its metabolites trigger the plant “cry for help” response to assemble disease suppressing and growth promoting rhizomicrobiome[J]. Nature Communications, 2024, 15(1): 1907.
[75] Li J, Wu L, Zhou Y, et al. Kobresia humilis via root-released flavonoids recruit Bacillus for promoted growth[J]. Microbiological Research, 2024, 287: 127866.
[76] Guo L, Zhang X, Zhao J, et al. Enhancement of sulfur metabolism and antioxidant machinery confers Bacillus sp. Jrh14-10–induced alkaline stress tolerance in plant[J]. Plant Physiology and Biochemistry, 2023, 203: 108063.
[77] Abdelaziz A M, Hashem A H, El-Sayyad G S, et al. Biocontrol of soil borne diseases by plant growth promoting rhizobacteria[J]. Tropical Plant Pathology, 2023, 48(2): 105-127.
[78] Teixidó N, Usall J, Torres R. Insight into a successful development of biocontrol agents: production, formulation, packaging, and shelf life as key aspects[J]. Horticulturae, 2022, 8(4): 305.
[79] Stojanovic S S, Karabegovic I, Beskoski V, et al. Bacillus based microbial formulations: optimization of the production process[J]. Hemijska Industrija, 2019, 73(3): 169-182.
[80] 刘晓辉, 敖静, 高晓梅, 等. 一株拮抗多种土传病害病原菌的芽胞杆菌筛选鉴定及发酵工艺优化[J]. 微生物学杂志, 2025, 45(2): 70-79.
[81] 张晓云, 曲远航, 郭庆港, 等. 萎缩芽胞杆菌HMB22922发酵工艺及其制剂研制[J]. 中国生物防治学报, 2019, 35(5): 768-776.
[82] Sanchis V. From microbial sprays to insect-resistant transgenic plants: history of the biospesticide Bacillus thuringiensis: A review[J]. Agronomy for Sustainable Development, 2011, 31(1): 217-231.