Effects of Three Kinds of Ultraviolet Radiation on Pupae of Drosophila melanogaster

doi:10.16409/j.cnki.2095-039x.2021.01.016

Abstract

Abstract: The pupae of Drosophila melanogaster were irradiated by three kinds of ultraviolet lamps (UVA, UVB and UVC), and the effects of irradiation on the eclosion rate, sex ratio, flight rate, dead pupa rate, adult dry weight, pupa weight, F1 eclosion rate, F1 sex ratio, F1 dead pupa rate and F1 adult dry weight were studied. The results showed that the pupa emergence rate of D. melanogaster was significantly reduced after irradiation, among which the pupa emergence rate of D. melanogaster exposed to UVB for 6 h was the lowest (10.00%). The average sex ratio of 9 h group after UVA irradiation was 2.32, which was significantly higher than that of other treatment groups. After UVB irradiation, the pupa emergence rate of D. melanogaster decreased significantly, among which the pupa emergence rate of D. melanogaster was as low as 4.00% after 6 h of UVB treatment. The death rate of pupae in the group exposed to UVB was significantly higher than that in the control group and other groups, and the average death rate of pupae in the group exposed to UVB for 6 h reached 89.33% and the highest was 94.00%. After UV irradiation, pupa weight of each group increased significantly compared with that of the control group. After UVB and UVC irradiation, the dry weight of adult D. melanogaster was significantly lower than that of the control group. After UV irradiation, the dry weight of D. melanogaster F1 adult decreased compared with the control group, and the difference was significant. This study can provide reference for UV mutagenesis and biological control of insect pests.

Key words: Drosophila melanogaster, ultraviolet, irradiation

CLC Number:

S433

LIU Xuxiang, YI Chuandong, AO Guofu, CHEN Shi, JI Qinge. Effects of Three Kinds of Ultraviolet Radiation on Pupae of Drosophila melanogaster[J]. Chinese Journal of Biological Control, 2021, 37(2): 228-234.

References

[1] 钱远槐, 刘艳玲, 李守涛, 等. 中国黑腹果蝇种组的组成与分布[J]. 湖北大学学报(自科科学版), 2006, 28(4):397-402.
[2] 张开春, 闫国华, 郭晓军, 等. 斑翅果蝇(Drosophila suzukii)研究现状[J]. 果树学报, 2014, 31(4):717-721.
[3] 蔡普默, 向候君, 仪传冬, 等. 斑翅果蝇危害健康水果机理研究进展[J]. 江西农业大学学报, 2017, 39(2):295-301.
[4] Kanzawa T. Studies on Drosophila suzukii Mats[M]. Kofu, Yamanashi Agricultural Experimental Station, 1939, 49.
[5] 刘素宁, 沈杰. 果蝇基因组与功能基因研究进展[J]. 应用昆虫学报, 2011, 48(6):1559-1572.
[6] Chu F, Borthakur A, Sun X, et al. The Siva-1 putative amphipathic helical region (SAH) is sufficient to bind to BCLXL and sensitize cells to UV radiation induced apoptosis[J]. Apoptosis, 2004(9):83-95.
[7] Ravi D, Muniyappa H, Das K C. Caffeine inhibits UV mediated NF-kB activation in A2058 melanoma cells:An ATM-PKCδ-p38 MAPK-dependent mechanism[J]. Molecular and Cellular Biochemistry, 2008(308):193-200.
[8] 钱晓薇, 吴秀毅, 林国栋, 等. 紫外线对小白鼠免疫遗传的损伤效应[J]. 中国细胞生物学学报, 2010, 32(2):277-280.
[9] 杨丽丽, 尹宝华, 侯文菊, 等. 芽孢杆菌诱变育种研究进展[J]. 山西农业科学, 2012, 40(7):804-806.
[10] 谌斌. 紫红曲的原生质体紫外线诱变育种[J]. 广西科学院学报, 1999(2):40-42.
[11] 曹恩华. UVA的辐射效应及其分子机理[J]. 激光生物学报, 1994, 3(4):529-534.
[12] 倪建, 华周华, 戴修道. 长波紫外线对人皮肤成纤维细胞DNA的损伤[J]. 中华劳动卫生职业病杂志, 2002, 20(6):471.
[13] 郑俊丽. UVA照射对黑腹果蝇生物学特性和抗氧化反应的影响[D]. 武汉:华中农业大学, 2011.
[14] 张俊贤, 郭光艳, 齐志广. 紫外线对果蝇生长发育和表型变异的影响[J]. 河北师范大学学报, 2006, 30(1):90-93.
[15] 应琼琼, 刘婷, 顾蔚. 紫外线照射对果蝇凋亡相关基因reaper、grim、hid表达的影响[J]. 中国老年学杂志, 2012, 32(11):2307-2309.
[16] 林清彩. 铃木氏果蝇和黑腹果蝇生态学特性及化学防治研究[D]. 济南:山东农业大学, 2015.
[17] 刘旭祥, 敖国富, 林嘉, 等. 一种黑腹果蝇蛹的简易大量繁殖方法[P]. CN110742024A, 2020-02-04.
[18] Iltefat H, Alexis B, Indermeet K, et al. Ultraviolet germicidal irradiation:Possible method for respirator disinfection to facilitate reuse during the COVID-19 pandemic[J]. Elsevier Inc., 2020, 82(6):1511-1512.
[19] Sahar A. Effect of chemical, microwave irradiation, steam autoclave, ultraviolet light radiation, ozone and electrolyzed oxidizing water disinfection on properties of impression materials:A systematic review and meta-analysis study[J]. Elsevier B.V., 2020, 32(4):161-170.
[20] 李江. 紫外线消毒技术的研究[D]. 天津:天津大学, 2004.
[21] 赵贝. UV-B长期胁迫对麦长管蚜种群参数、表皮及几丁质合成酶的影响研究[D]. 杨凌:西北农林科技大学, 2019.
[22] 桑文. 赤拟谷盗与四纹豆象对物理因子胁迫的响应机制研究[D]. 武汉:华中农业大学, 2016.
[23] 王丹. 果蝇MBF1通过调控代谢相关基因表达响应冷胁迫[D]. 济南:山东农业大学, 2016.
[24] 唐玉龙. 紫外辐射对DNA损伤的拉曼光谱研究[D]. 广州:华南师范大学, 2005.
[25] 张昕, 范立英. 紫外线杀菌机理及在食品工业中应用[J]. 吉林粮食高等专科学校学报, 1996(1):23-25.
[26] 钟兰军. 不同剂量紫外线照射对宁德晚熟龙眼水南1号保鲜效果的影响[J]. 福建农业科技, 2019(12):25-30.
[27] Mustafa P, Mustafa K. Detection of irradiated black tea (Camellia sinensis) and rooibos tea (Aspalathus linearis) by ESR spectroscopy[J]. Food Chemistry, 2007, 107(2):956-961.
[28] 袁伟宁, 朱仰艳, 孙小玲, 等. 紫外线胁迫对红色型豌豆蚜生物学特性的影响[J]. 植物保护, 2016, 42(4):77-82.
[29] 张雅君, 梁佳勇, 曾慕衡. 紫外线对雄果蝇寿命及子代生理的影响[J]. 天津农业科学, 2013, 19(7):75-78.
[30] Wen H W, Hsieh M F, Wang Y T, et al. Application of gamma irradiation in ginseng for both photo degradation of pesticide pentachloronitrobenzene and microbial decontamina-tion[J]. Journal of Hazardous Materials, 2010, 176(1-3):280-287.
[31] Gowton C M, Reut M, Carrillo J. Peppermint essential oil inhibits Drosophila suzukii emergence but reduces Pachycrepoideus vindemmiae parasitism rates[J]. Scientific Reports, 2020, 10:9090.
[32] Yang L, Yang Y, Liu M M, et al. Identification and comparative analysis of venom proteins in a pupal ectoparasitoid, Pachycrepoideus vindemmiae[J]. Frontiers in physiology, 2020, 11:9.
[33] Wang X G, Kaçar G, Biondi A, et al. Life-history and host preference of Trichopria drosophilae, a pupal parasitoid of spotted wing drosophila[J]. BioControl, 2016, 61(4):387-397.
[34] Stacconi M V R, Grassi A, Ioriatti C, et al. Augmentative releases of Trichopria drosophilae for the suppression of early season Drosophila suzukii populations[J]. BioControl, 2019, 64(1):9-19.