Effect of Bemisia tabaci Population Density on Spreading of Tomato Yellow Leaf Curl Virus and Screening of High-efficiency Insecticidal Fungus

doi:10.16409/j.cnki.2095-039x.2024.11.012

Abstract

Abstract: Bemisia tabaci is the only vector of tomato yellow leaf curl virus (TYLCV), which can cause plant growth retardation or stagnation and affect the yield and quality of tomato seriously. In order to find out the correlation between population density of B. tabaci and the degree of TYLCV occurrence and obtain a high-efficiency insecticidal fungus strain against B. tabaci so as to control the TYLCV effectively, the effects of different population densities of B. tabaci on the transmission of TYLCV were studied by real-time fluorescent quantitative PCR technology. The control efficiency of different fungal strains on B. tabaci were tested by laboratory bioassay and field trail. The results showed that the relationship between relative content of TYLCV in tomato plants infested with B. tabachi for 9 days and the population density of B. tabaciis fitted well with the logistic curve model (R²＝ 0.9558). When the density of B. tabaci was higher than 400 (148/m³), the expression of virus gene in tomato increased rapidly. The 12 entomopathogenic fungi strains were tested in laboratory for their control of B. tabaci, which showed that Beauveria bassiana IPPBKN001 had the best control after 5 days of treatment, with corrected mortality of81.11%. The results of field trail showed that the control efficiency of IPPBKN001 was over 76.36% at 5 d post application. In this study, the tomato yellow leaf curve virus disease is closely correlated to the population density of B. tabaci and an efficient B. bassiana strain against B. tabaci is obtained, which provides the microbial resources for the control of tomato virus disease.

Key words: Bemisia tabaci, tomato yellow leaf curl virus, population density, entomopathogenic fungi

CLC Number:

S476.1

FANG Wenjiao, WANG Zifan, HAO Xinyu, JIA Hongwei, WANG Xiaoling, LIU Tinghui, TU Xiongbing, FENG Shiqian, SHI Jianhua, WANG Guangjun. Effect of Bemisia tabaci Population Density on Spreading of Tomato Yellow Leaf Curl Virus and Screening of High-efficiency Insecticidal Fungus[J]. Chinese Journal of Biological Control, 2025, 41(3): 702-709.

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