Biological Characteristics and Pathogenicity of Four Dominant Pathogenic Fungi of Overwintering Larvae of Ostrinia furnacalis in Xinjiang Desert Oasis Region

doi:10.16409/j.cnki.2095-039x.2023.03.002

Abstract

Abstract: The taxonomic status, biological characteristics and biocontrol potential of four dominant pathogenic fungi isolated from naturally diseased overwintering Ostrinia furnacalis larvae in Xinjiang desert oasis area were determeind. The strains were identified as Aspergillus nomius, Beauveria bassiana, Penicillium raistrickii, and Lecanicillium attenuatum. The colony growth rate of A. nomius was 10.10 mm/d, the spore germination rate was 96.83% at 48 h, the spore production was 1.84×10¹⁰ spores/mL at 14 d. A. nomius had the strongest virulence to the third instar larvae of O. furnacalis, with LT₅₀ and LC₅₀ of 4.16 d and 4.283×10² spores/mL, respectively, and a 7-d cumulative corrected mortality rate of 93.94%. B. bassiana came second in virulence, with LT₅₀ and LC₅₀ of 5.03 d and 1.724×10⁴ spores/mL, respectively, and a 7-d cumulative mortality rate of 73.48%. With LT₅₀, LC₅₀ and a 7-d cumulative mortality rate of 5.04 d, 1.829×10⁴ spores/mL, and 69.80%, respectively, L. attenuatum showed no significant virulence than B. bassiana, P. raistrickii had low virulence to O. furnacalis, with LT₅₀, LC₅₀ and 7-d cumulative mortality rate of 6.04 d, 4.062×10⁵ spores/mL, and 53.03%, respectively. When inoculated with 1×10⁸ spores/mL A. nomius spore suspension for 2 days, the 3rd instar larvae of O. furnacalis showed slow movement, no feeding and death; black-brown spots began to appear on the rear part, the whole body gradually dried up, and at 7 d since inoculation yellow-green hyphae grew on the insects and a large number of spores produced from the insects. In summary, A. nomius has strong pathogenicity and high mortality rate for the 3rd instar larvae of O. furnacalis and is of great application potential.

Key words: Ostrinia furnacalis, entomopathogenic fungi, biological characteristics, pathogenicity, biological control

CLC Number:

WANG Xiaowu, JIANG Xudong, DING Xinhua, YUAN Zihan, FU Kaiyun, JIA Zunzun, TOULXUN Ahemaiti, GUO Wenchao. Biological Characteristics and Pathogenicity of Four Dominant Pathogenic Fungi of Overwintering Larvae of Ostrinia furnacalis in Xinjiang Desert Oasis Region[J]. Chinese Journal of Biological Control, 2023, 39(2): 305-312.

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