Identification of Fusarium equiseti and Its Pathogenicity to Myzus persicae

doi:10.16409/j.cnki.2095-039x.2022.07.002

Abstract

Abstract: A strain of entomopathogenic fungus was isolated from the corpse of Myzus persicae and designated as JMF-01. In this study, the author aims to determine the taxonomic status of the strain and explore its biocontrol potential. The strain was isolated and purified from the diseased peach aphid corpse. Based on morphological observation and rDNA-ITS, RPB2 and IGS sequence analysis, the phylogenetic tree was constructed to identify the strain. The pathogenicity of the strain to M. persicae under different spore suspension concentrations was measured by leaf dipping and insect dipping method and the control of the aphids by the strain was determined in greenhouse tests. The results showed that the colony diameter of JMF-01 was 58―60 mm at 5 days on PDA medium, conidia were sickle-shaped and separated by 3―7. Strain JMF-01 showed strong virulence to M. persicae and the cumulative corrected mortality and LC₅₀ reached 85% and 9.87×10⁵ cfu/mL at 7 days after treatment, respectively. The control efficiency of the aphids by the spore suspension at the highest concentration in greenhouse was higher than 70% at 14 days after treatment. The rDNA-ITS sequence (MW404610) of the strain was 100% consistent with that of Fusarium equiseti (GenBank accession number: JF773657), locating in the same branch of the phylogenetic tree; the similarity of RPB2 and IGS sequences with F. equiseti (GenBank accession number: MK077112, KX583611) also reached more than 99%, clustering in the same branch of the phylogenetic tree. In conclusion, the strain JMF-01 identified as F. equiseti is a virulent pathogen of M. persicae with high biocontrol potential.

Key words: Myzus persicae, Fusarium equiseti, entomopathogenic fungi, pathogenicity

CLC Number:

S476.1

ZHAO Xueyi, CHAI Junfa, ZHANG Xuanyi, HONG Bo, JIA Yanxia. Identification of Fusarium equiseti and Its Pathogenicity to Myzus persicae[J]. Chinese Journal of Biological Control, 2023, 39(1): 69-76.

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