Screening of Antagonistic Bacteria and Its Control Effect on Cotton Stiff Bolls

doi:10.16409/j.cnki.2095-039x.2025.02.039

Abstract

Abstract: The cotton stiff bolls disease caused by Cladosporium spp. occurs seriously in various cotton-growing areas in Xinjiang. This study utilized the soil dilution method and plate standoff technique to screen and obtain three strains of bacteria with excellent antagonistic effects against Cladosporium spp. to prevent and control cotton stiff bolls disease in a green and safe way. The results of the mycelial inhibition test showed that WHZ-3, WHZ-60, and QHZ-42 showed significant inhibition on the growth of Cladosporium spp., which inhibited the growth of mycelium of Cladosporium cladosporiodes by 64.70%, 60.57% and 75.63%, and that of Cladosporium limoniforme by 63.92%, 61.45% and 74.15%, and the pathogen mycelium appeared to be deformity of the mycelium of the pathogen. The results of the indoor test showed that the three strains of antagonistic bacteria inhibited 63.99%, 27.70% and 90.25% of Cladosporium cladosporiodes and 43.62%, 75.92% and 82.83% of Cladosporium limoniforme. The results of the field test showed that except for WHZ-60, the field preventive effect of strains WHZ-3 and QHZ-42 on cotton stiff bolls disease was equal to or higher than that of the control agent 30% pyraclostrobin by 64.34%, 67.75%, and 80.16%, respectively. The results of the inhibition spectrum showed that the three strains of antagonist bacteria were able to inhibit the growth of various pathogenic fungi such as Verticillium dahliae, Alternaria solani and Bipolaris sorokiniana. After morphological observation, physiological and biochemical characterization, and combined with 16S rDNA and rpoB multi-gene sequence analysis, WHZ-3, WHZ-60, and QHZ-42 were identified as salt-tolerant Bacillus halotolerans, Bacillus mojavensis and Bacillus subtilis, respectively. The results of this study provide potentially valuable biocontrol strains for the control of cotton stiff bolls disease.

Key words: cotton stiff bolls, Cladosporium spp., biological control, control effect

CLC Number:

S476

ZHANG Chi, JIN Bingkun, LI Jingwen, DU Yejuan, HUANG Jiafeng, ZHANG Xuekun, LIU Zheng. Screening of Antagonistic Bacteria and Its Control Effect on Cotton Stiff Bolls[J]. Chinese Journal of Biological Control, 2025, 41(4): 895-905.

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