Effects of Combined Application of Benzothiadiazole and Trichoderma viride on Growth and Root Rot Occurrence of Panax notoginseng

doi:10.16409/j.cnki.2095-039x.2025.02.073

Abstract

Abstract: This study systematically evaluated the effects of synergistic treatment with foliar benzothiadiazole (BTH) application and root inoculation of beneficial Trichoderma viride on growth and disease resistance of Panax notoginseng, aiming to provide novel strategies and approaches for green control of replant failure in P. notoginseng cultivation. The results showed that T. viride significantly inhibited the root rot pathogens Plectosphaerella cucumerina (P) and Cylindrocarpon destructans (RS6), with inhibition rates exceeding 58%, while BTH alone had no significant inhibitory effect on the growth of root rot pathogens. Pot experiment demonstrated that T. viride could stably colonize the rhizosphere of P. notoginseng. When co-applied with BTH treatment, this combination significantly reduced the incidence of root rot disease to 37.67%, increased the seedling survival rate by 10.62%, and enhanced the fresh weight by 0.34 g. Notably, 0.6 mmol/L BTH was identified as the optimal concentration, achieving a maximum control efficiency of 55.29% against root rot. Furthermore, combined treatment with 0.4 mmol/L BTH and T. viride significantly increased the content of saponins, including Rd, R1, Rb₁, and the total content of five major saponins (Re+Rd+R1+Rb₁+Rg₁). In conclusion, the synergistic application of foliar BTH spray and root T. viride inoculation significantly promoted the growth and saponin accumulation of P. notoginseng, enhanced its disease resistance, and effectively alleviated root rot occurrence. These findings provided theoretical evidence for the ecological control of continuous cropping obstacles of P. notoginseng and other crop diseases.

Key words: Panax notoginseng, benzothiadiazole, Trichoderma viride, root rot disease

CLC Number:

S476

YAN Xiubei, LI Gaihui, LUO Lifen, ZHAO Sihan, JIN Shuangting, YIN Tong, ZHU Shusheng, YANG Min. Effects of Combined Application of Benzothiadiazole and Trichoderma viride on Growth and Root Rot Occurrence of Panax notoginseng[J]. Chinese Journal of Biological Control, 2026, 42(1): 44-54.

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