Potential of Scallion Rotation to Improve Soil Microbial Community and Alleviate Continuous Cropping Obstacle of Panax notoginseng

doi:10.16409/j.cnki.2095-039x.2022.03.009

Abstract

Abstract: The effect of scallion rotation with Panax notoginseng on microbes in soils continuously cultivated with P. notoginseng was evaluated to provide evidence for alleviating the continuous cropping obstacle of P. notoginseng by crop rotation. Scallions were cultivated two times in a row on the pre-harvested P. notoginseng soil, while the control soil was left fallow. The soil microbial diversity, composition and key microorganisms in soils rotated with scallion were measured using sequencing bacterial 16S rRNA and fungal ITS region. Then, the key culturable bacteria Pseudomonas were isolated and tested for their antagonistic function against P. notoginseng root rot pathogens. The results showed that scallion rotation significantly improved the richness of soil bacteria and fungi. The bacteria and fungi from the rotation treatment and the control clearly separated on the PCoA plot. For bacteria, the relative abundance of Proteobacteria was significantly increased, while Acidobacteria, Chloroflexi, etc. were significant decreased at the phylum level. With respect to fungi, the relative abundance of Ascomycota was significantly suppressed, and Basidiomycota was significantly increased after scallion rotation. LEfSe analysis showed that the key bacteria, Proteobacteria and its subordinate Pseudomonas were significantly increased after scallion rotation, while the abundance of Fusarium and Ilyonectria, which causes root rot of P. notoginseng, decreased after rotation. Twenty-two strains of Pseudomonas were isolated from the scallion rotation soil, and 15 strains of P. aeruginosa had a good antagonistic effect on P. notoginseng root rot pathogens. In conclusion, scallion rotation can significantly improve the microbial status of soil continuously cropped with P. notoginseng, increase the relative abundance of beneficial bacteria and reduce root rot pathogens. The rotation system of scallion and P. notoginseng has some potential to alleviate the continuous cropping obstacle of P. notoginseng.

Key words: Panax notoginseng, scallion, crop rotation, soil microbe, Pseudomonas

CLC Number:

S432.1

LIU Haijiao, ZUO Denghong, XU Jie, SU Yingwei, ZHU Shusheng, YANG Min. Potential of Scallion Rotation to Improve Soil Microbial Community and Alleviate Continuous Cropping Obstacle of Panax notoginseng[J]. Chinese Journal of Biological Control, 2022, 38(6): 1473-1483.

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