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Chinese Journal of Biological Control ›› 2023, Vol. 39 ›› Issue (6): 1398-1407.DOI: 10.16409/j.cnki.2095-039x.2023.02.063

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Screening of Antagonistic Isolate Bacillus velezensis Bv1-4 and Its Control Effect against Root Rot Disease on Salvia miltiorrhiza

WANG Fei1, YANG Jin1, LI Shaojian1, LI Xuemeng1, WEN Yi1, ZHAO Ying2, QIN Yanhong1, GAO Suxia1, LIU Yuxia1, LU Chuantao1   

  1. 1. Institute of Plant Protection, Henan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Zhengzhou 450002, China;
    2. College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China
  • Received:2023-05-11 Online:2023-12-08 Published:2023-12-20

Abstract: Root rot of Salvia miltiorrhiza is a common root disease caused by Fusarium spp., which has become one of the primary diseases affecting the production of S. miltiorrhiza. In order to screen effective biocontrol bacteria against root rot disease of S. miltiorrhiza, a biocontrol bacteria Bv1-4 with significant antagonism was isolated from the rhizosphere soil of healthy S. miltiorrhiza by the plate confrontation test. Based on morphological, physiological and biochemical characteristics, and sequence analysis, isolate Bv1-4 was identified as Bacillus velezensis. The inhibition rates of Bv1-4 against root rot of S. miltiorrhiza were 65.54%, 68.28%, and 66.87% for F. proliferatum, F. solani, and F. oxysporium, respectively. Further inhibition spectrum tests showed that Bv1-4 had a broad range of antifungal activity against various plant pathogenic fungi, such as Macrophomina phaseoli, Sclerotium rolfsii, Altrnaria tenuissima, and others. The results of the potted experiment proved that the fresh weight of above- and below-ground parts of S. miltiorrhiza after inoculation with Bv1-4 was significantly increased compared with the control group, and the increase rate was 70.97% and 70.37%, respectively; the control effect could reach more than 80% after mixed inoculation with Bv1-4 and three pathogens of S. miltiorrhiza root rot. In conclusion, Bv1-4 has good biocontrol characteristics and has good application potential in the biocontrol of plant diseases.

Key words: Bacillus velezensis, Salvia miltiorrhiza, root rot disease, biological control

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