Detoxification Effect of Providencia rettgeri in the Gut of Spodoptera frugiperda on Chlorantraniliprole

doi:10.16409/j.cnki.2095-039x.2025.09.022

Abstract

Abstract: Chlorantraniliprole(CAP) is one of the commonly employed chemical agents for controlling fall armyworm(FAW). Long-term use will cause the fall armyworm to develop resistance to chlorantranamide, reducing the efficacy of the insecticide. To explore the role of gut microbiota in the process of resistance development, this study compared the differences in intestinal microbiota before and after the intake of chlorantraniliprole, screened and isolated the differential gut microbiota, and conducted bioassay tests. Three types of bacteria were screened and isolated from fall armyworm midgut and cultured on LB by in vitro culture method. Biotests revealed that the strains could reduce the virulence of chlorantraniliprole and significantly reduce the mortality rate of the fall armyworm. The amounts of chlorantraniliprole before and after mixing with the strains were analyzed by LC-MS. It was found that the strains could degrade chlorantraniliprole, with a rate reaching 42%. Further genomic sequencing and phylogenetic analysis identified the strain as Providencia rettgeri, with a genome size of 4391370 bp encoding 3985 genes. In conclusion, P. rettgeri can increase the resistance of fall armyworm to chlorantraniliprole by directly degrading it, providing a basis for further understanding the mechanism on developing resistance to chlorantraniliprole.

Key words: detoxification, Providencia rettgeri, chlorantraniliprole, Spodoptera frugiperda

CLC Number:

S435.6

CHEN Feng, ZHANG Juntao, WANG Xiuping, ZHANG Yongjun, ZHANG Tiantao. Detoxification Effect of Providencia rettgeri in the Gut of Spodoptera frugiperda on Chlorantraniliprole[J]. Chinese Journal of Biological Control, 2026, 42(4): 809-817.

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