Effects of Seed Soaking with Beauveria bassiana and Metarhizium rileyi on Defense Enzyme Activities and Insect Resistance in Maize Leaves

doi:10.16409/j.cnki.2095-039x.2025.01.002

Abstract

Abstract: This study aim is to delve into the impact of seed treatment using entomopathogenic fungi Beauveria bassiana Bb20091317 and Metarhizium rileyi MrCDTLJ1 on insect resistance and the activity of defense enzymes in maize. Employing a pot experiment setup, the investigation aimed to evaluate the resistance of maize leaves which seeds soaked with B. bassiana Bb20091317 and M. rileyi MrCDTLJ1, respectively, against Ostrinia furnacalis and Spodoptera frugiperda, as well as the activity of levels of key enzymes such as phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), superoxide dismutase (SOD), and peroxidase (POD) in S. frugiperda after injured maize leaves over various time intervals (0, 2, 12, 24, and 48 hours). The findings revealed that treated seeds with Bb20091317 significantly blostered the activities of POD, PPO, and SOD in maize leaves, both before and after the induction of S. frugiperda damage. Similarly, soaked seeds with MrCDTLJ1 significantly elevated POD and SOD activities in maize leaves before and after S. frugiperda damage, but did not significantly induce SOD and PAL activities. Notably, the weight of O. furnacalis larvae feeding on the leaves which seeds treated with Bb20091317 and MrCDTLJ1 was significantly lower compared to those feeding on non-soaked maize leaves. Additionally, the weight of S. frugiperda larvae induced by Bb20091317 and MrCDTLJ1-treated seeds, coupled with 24 hours of leaf damage, was significantly lower than larvae from untreated maize leaves subjected to S. frugiperda damage. However, there was no significant difference in the weight of larvae from S. frugiperda induced maize leaves for 24 hours compared to control leaves. In essence, the seed treatments with B. bassiana and M. rileyi stimulated systemic defense responses in maize, as evidenced by heightened enzyme activation following S. frugiperda herbivory, consequently enhancing resistance against O. furnacalis and S. frugiperda.

Key words: Beauveria bassiana, Metarhizium rileyi, Zea mays, Ostrinia furnacalis, Spodoptera frugiperda, defense enzymes

CLC Number:

S476.1

ZHAN Zhaofeng, GOU Xuelian, WANG Zhenying, ZHANG Yongjun, WANG Xiuping, GUO Jingfei. Effects of Seed Soaking with Beauveria bassiana and Metarhizium rileyi on Defense Enzyme Activities and Insect Resistance in Maize Leaves[J]. Chinese Journal of Biological Control, 2025, 41(1): 54-62.

References

[1] 王振营, 王晓鸣. 我国玉米病虫害发生现状、趋势与防控对策[J]. 植物保护, 2019, 45(1): 1-11.
[2] Jing D P, Guo J F, Jiang Y Y, et al. Initial detections and spread of invasive Spodoptera frugiperda in China and comparisons with other noctuid larvae in cornfields using molecular techniques[J]. Insect Science, 2020, 27(4): 780-790.
[3] 郭井菲, 赵建周, 何康来, 等. 警惕危险性害虫草地贪夜蛾入侵中国[J]. 植物保护, 2018, 44(6): 1-10.
[4] 郭井菲, 张永军, 王振营, 等. 中国应对草地贪夜蛾入侵研究的主要进展[J]. 植物保护, 2022, 48(4): 79-87.
[5] 程东美, 洪婉雯, 孙辉, 等. 草地贪夜蛾幼虫僵虫发生率调查及致病菌分离鉴定[J]. 环境昆虫学报, 2020, 42(6): 1298-1304.
[6] 胡本进, 李昌春, 徐丽娜, 等. 亚洲玉米螟高致病性球孢白僵菌菌株筛选及田间防治试验[J]. 中国植保导刊, 2013, 33(8): 23-26.
[7] 徐艳聆, 吕文彦, 杜开书, 等. 亚洲玉米螟优良球孢白僵菌菌株的筛选[J]. 中国农学通报, 2010, 26(21): 278-281.
[8] 秦华伟, 姚嘉静, 门兴元, 等. 玉米内生真菌对草地贪夜蛾生长发育的影响[J]. 植物保护, 2021, 47(5): 94-97.
[9] 庞继鑫, 温绍海, 杜广祖, 等. 一株侵染草地贪夜蛾成虫的球孢白僵菌的分离鉴定[J]. 植物保护, 2022, 48(1): 185-190, 203.
[10] 邹春华, 刘继辉, 吴伟, 等. 草地贪夜蛾病原白僵菌的分离鉴定及其致病力研究[J]. 植物保护, 2023, 49(6): 242-246.
[11] 毕思佳, 吴晨源, 胡本进, 等. 球孢白僵菌Bb378菌株的分离、鉴定及对草地贪夜蛾的致病性研究[J]. 应用昆虫学报, 2023, 60(4): 1223-1232.
[12] 胡飞, 徐婷婷, 彭辉, 等. 莱氏绿僵菌Mr006鉴定及对草地贪夜蛾致病力[J]. 中国生物防治学报, 2024, 40(1): 44-51.
[13] KuzhuppillymyalPrabhakarankutty L, FerraraRivero F H., TamezGuerra P, et al. Effect of Beauveria bassiana-seed treatment on Zea mays L. response against Spodoptera frugiperda[J]. Applied Sciences, 2021, 11(7): 2887-2887.
[14] 隋丽, 费泓强, 张正坤, 等. 白僵菌定殖对玉米苗期生长和防御酶指标的影响[C]//中国植物保护学会.病虫害绿色防控与农产品质量安全——中国植物保护学会2015年学术年会论文集. 中国农业科学技术出版社, 2015, 1.
[15] Diana L C, Keyan Z, Julissa M E, et al. The entomopathogenic fungal endophytes Purpureocillium lilacinum (formerly Paecilomyces lilacinus) and Beauveria bassiana negatively affect cotton aphid reproduction under both greenhouse and field conditions[J]. PLoS ONE, 2014, 9(8): e103891.
[16] Russo L M, Vianna F M, Scorsetti C A, et al. Entomopathogenic fungi as dual control agents against two phytopathogens and the lepidopteran pest Rachiplusia nu in soybean[J]. Journal of Fungi, 2024, 10(2): 93.
[17] Tscharntke T, Thiessen S, Dolch R, et al. Herbivory, induced resistance, and interplant signal transfer in Alnus glutinosa[J]. Biochemical Systematics and Ecology, 2001, 29(10): 1025-1047.
[18] Raufa B, Jawad M U, Wang Y Z, et al. Myco-synergism boosts herbivory-induced maize defense by triggering antioxidants and phytohormone signaling[J]. Frontiers in Plant Science, 2022, 13790504-790504.
[19] Raufa B, Gou X L, Dong H, et al. Endophytic fungi-mediated defense signaling in maize: unraveling the role of WRKY36 in regulating immunity against Spodoptera frugiperda[J]. Physiologia Plantarum, 2024, 176(2): e14243.
[20] 费泓强, 隋丽, 朱慧, 等. 球孢白僵菌在玉米苗期的定殖及其对玉米生理生化特性的影响[J]. 中国生物防治学报, 2016, 32(6): 721-727.
[21] 龙秀珍, 高旭渊, 曾宪儒, 等. 一株莱氏绿僵菌的筛选及其对草地贪夜蛾的毒力[J]. 中国生物防治学报, 2021, 37(6): 1111-1119.
[22] 卫秋阳. 球孢白僵菌诱导番茄对烟粉虱抗性研究[D]. 重庆: 西南大学, 2021.
[23] Xie J H, Chai T T, Xu R, et al. Induction of defense-related enzymes in patchouli inoculated with virulent Ralstonia solanacearum[J]. Electronic Journal of Biotechnology, 2017, 27: 63-69.
[24] 郭祖国, 王梦馨, 崔林, 等. 6种防御酶调控植物体应答虫害胁迫机制的研究进展[J]. 应用生态学报, 2018, 29(12): 4248-4258.
[25] 胡庆辉, 王程栋, 王树声, 等. NaCl胁迫下鲜烟叶中多酚物质含量及PAL和PPO活性变化[J]. 中国烟草科学, 2013, 34(1): 51-55.
[26] 从春蕾, 郅军锐, 廖启荣, 等. 蓟马取食、机械损伤以及外源水杨酸甲酯和茉莉酸对菜豆叶片防御酶活性的影响[J]. 昆虫学报, 2014, 57(5): 564-571.
[27] 帕提玛·乌木尔汗, 陈丽慧, 崔燕华, 等. 二斑叶螨危害对棉花应激防御酶活性的影响[J]. 环境昆虫学报, 2016, 38(6): 1185-1191.
[28] Gao J, Gong L F, Wang H H, et al. Expression and functional analysis of ace1 and ace2 reveal their differential roles in larval growth and insecticide sensitivity in Spodoptera frugiperda (J. E. Smith, 1797)[J]. Journal of Pest Science, 2023, 96(4): 1651-1666.
[29] 冯树丹, 李晓慧, 汪洋洲, 等. 二种交配型球孢白僵菌对亚洲玉米螟的生态控制作用[J]. 生态学报, 2017, 37(2): 650-658.
[30] 苟雪莲, 王振营, 涂雄兵, 等. 两株虫生真菌对草地贪夜蛾的致病力及高毒力菌株与卵寄生蜂的相容性[J]. 植物保护学报, 2022, 49(5): 1505-1512.
[31] 王芹芹, 崔丽, 王立, 等. 草地贪夜蛾对杀虫剂的抗性研究进展[J]. 农药学学报, 2019, 21(4): 401-408.
[32] Chen H L, Hasnain A, Cheng Q H, et al. Resistance monitoring and mechanism in the fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) for chlorantraniliprole from Sichuan Province, China[J]. Frontiers in Physiology, 2023, 14: 1180655.
[33] 周绍群. 不同玉米品种对草地贪夜蛾抗性的评估及其在防治中的应用前景[J]. 植物保护, 2019, 45(5): 8-12.