Effect of Chaetomium globosumCEF-082 on Eggplant Verticillium Wilt

doi:10.16409/j.cnki.2095-039x.2026.02.052

Abstract

Abstract: Eggplant Verticillium wilt, caused by the soil-borne fungus Verticillium dahliae, is a devastating vascular disease that severely reduces yield and quality, necessitating the development of safe and efficient green control strategies. This study aimed to evaluate the biocontrol potential of Chaetomium globosumCEF-082, an endophytic fungus originally isolated from cotton, against eggplant Verticillium wilt and to preliminarily explore its effects on soil microecology. The in vitro antagonistic activity of CEF-082 against the pathogen was determined using confrontation culture, fungicide-containing medium, and spore germination assays. The control efficacy was assessed through pot and field experiments with root drenching applications of spore suspension and fermentation broth. Additionally, soil physicochemical properties, enzyme activities, and microbial community structure were analyzed. The results showed that CEF-082 significantly inhibited the mycelial growth and spore germination of V. dahliae. After 9 days of confrontation culture, the pathogen growth rate was reduced by 75.49% compared with the control, and the 10% fermentation broth achieved a spore germination inhibition rate of 70.63%. In pot experiments, the spore suspension treatment exhibited a control efficacy of 46.95%, while in field trials, the efficacy reached 53.46% at 40 d after treatment, which was comparable to that of the chemical fungicide carbendazim. Soil microecological analysis revealed that CEF-082 application significantly increased soil organic matter and available nutrient contents, enhanced the activities of sucrase, catalase, and urease, and improved the species richness and diversity of both bacterial and fungal communities, while simultaneously reducing the abundance of potential soilborne pathogens such as Fusarium spp. In conclusion, Chaetomium globosum CEF-082 possesses both direct antifungal activity and the capacity to improve soil health, making it a promising biocontrol resource for the green management of eggplant Verticillium wilt.

Key words: eggplant Verticillium wilt, Chaetomium globosum, biological control, Verticillium dahliae, soil microecology

CLC Number:

S476

LI Wenyan, WEI Feng, ZHANG Yalin, ZHAO Lihong, ZHOU Jinglong, HAN Jiangping, SONG Yong, FENG Hongjie, FENG Zili, ZHU Heqin. Effect of Chaetomium globosumCEF-082 on Eggplant Verticillium Wilt[J]. Chinese Journal of Biological Control, 2026, 42(5): 1157-1169.

/ Recommend

References

[1] 王锦秀.《植物名实图考》中一些百合科植物考证兼论茄子在中国的栽培起源和传播[D].北京:中国科学院研究生院(植物研究所), 2005.
[2] 国家统计局.中国统计年鉴[M].北京:中国统计出版社, 2020.
[3] 韩顺国.茄子黄萎病及防治研究[J].种子科技, 2022, 40(15):106-108.
[4] Hashimoto K. Studies on Verticillium wilt of eggplant[J]. Bulletin of Saitama Horticultural Experiment Station, 1989, 2(3):110.
[5] 张琳,张紫溪,陈秀,等.双酰胺类农药生物活性、生态毒性及残留行为研究进展[J].农药学学报, 2023, 25(2):295-309.
[6] 代京莎,李安章,朱红惠.粘细菌在植物病害生物防治中的作用[J].生物技术进展, 2016, 6(4):229-234.
[7] 危潇,曹春霞,黄大野,等.木霉菌生防作用机制及协同防病的研究进展[J].中国农业科技导报, 2024, 26(11):126-135.
[8] 赵欣,王怡霏,王嘉嘉,等.木霉菌对作物及土壤生态环境的影响[J].中国农业科技导报, 2023, 25(11):166-172.
[9] 梁宏,黄静,赵佳,等.生物防治棉花黄萎病的研究进展[J].生物技术通报, 2015, 31(5):1-6.
[10] Mendes R, Garbeva P, Raaijmakers J M. The rhizosphere microbiome:significance of plant beneficial, plant pathogenic, and human pathogenic microorganisms[J]. FEMS Microbiology Reviews, 2013, 37(5):634-663.
[11] 冯超红,李丽娟,张姣姣,等.球毛壳菌促生防病机制及应用研究进展[J].中国生物防治学报, 2023, 39(4):961-969.
[12] Kumar R, Kundu A, Dutta A,et al. Chemo-profiling of bioactive metabolites from Chaetomium globosum for biocontrol of Sclerotinia rot and plant growth promotion[J]. Fungal Biology, 2020, 125(3):167-176.
[13] Zhao, S S, Lin, C Y, Cheng, M, et al. New insight into the production improvement and resource generation of chaetoglobosin A in Chaetomium globosum[J]. Microbial Biotechnology, 2022, 15:2562-2577.
[14] Khattab A. Endophyte Chaetomium globosum improves the growth of maize plants and induces their resistance to late wilt disease[J]. Journal of Plant Diseases and Protection, 2022, 129:1125-1144.
[15] 渠非.木瓜海棠内生球毛壳菌MG2对苹果树腐烂病的生防作用研究[D].杨凌:西北农林科技大学, 2022.
[16] 冯超红,徐飞,张姣姣,等.小麦根部球毛壳菌的分离鉴定及其对全蚀病的生防作用[J].河南农业科学, 2025, 54(11):91.
[17] Kumari, S, Kumari, S, Attri, C, et al. GC-MS Analysis, Antioxidant and antifungal studies of different extracts of Chaetomium globosum isolated from Urginea indica[J]. BioMed Research International, 2022, 2022(1):1388850.
[18] 李文燕,魏锋,张亚林,等.茄子土传病害病原菌鉴定及球毛壳菌CEF-082对其的防治作用[J].中国农学通报, 2025, 41(24):111-118.
[19] 缪其松,王强,王东升,等.四种砧木对黄萎病高发区设施连作茄子产量、品质及发病率的影响[J].北方园艺, 2020(1):50-56.
[20] 沈其益.棉花病害-基础与防治[M].北京:科学出版社, 1992.
[21] 李义华,刁红亮,周稳稳,等.碳源对玫烟色虫草菌丝生长、芽生孢子产生及其耐热性的影响[J].菌物学报, 2022, 41(1):51-58.
[22] Kinkel L L, Bakker M G, Schlatter D C. A coevolutionary framework for managing disease-suppressive soils[J]. Annual Review of Phytopathology, 2011, 49(1):47-67.
[23] Liu X, Zhang S, Jiang Q, et al. Using community analysis to explore bacterial indicators for disease suppression of tobacco bacterial wilt[J]. Scientific Reports, 2016, 6(1):36773.
[24] Raaijmakers J M, Paulitz T C, Steinberg C, et al. The rhizosphere:a playground and battlefield for soilborne pathogens and beneficial microorganisms[J]. Plant and Soil, 2009, 321(1/2):341-361.
[25] Classen A T, Sundqvist M K, Henning J A, et al. Direct and indirect effects of climate change on soil microbial and soil microbial-plant interactions:What lies ahead?[J]. Ecosphere, 2015, 6(8):1-21.
[26] She S Y, Niu J J, Zhang C, et al. Significant relationship between soil bacterial community structure and incidence of bacterial wilt disease under continuous cropping system[J]. Archives of Microbiology, 2017, 199:267-275.
[27] Janvier C, Villeneuve F, Alabouvette C, et al. Soil health through soil disease suppression:which strategy from descriptors to indicators?[J]. Soil Biology and Biochemistry, 2007, 39(1):1-23.
[28] Nannipieri P, Ascher J, Ceccherini M T, et al. Microbial diversity and soil functions[J]. European Journal of Soil Science, 2003, 54(4):655-670.
[29] Carrasco F, Miranda V, Sede S, et al. Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae[J]. Arid Land Research and Management, 2023.
[30] Khattak A A, Chen X, Abdalamer I S, et al.Isolation and characterization of Bacillus velezensis:investigating its mechanisms as an effective biocontrol agent against Verticillium wilt in eggplants[J]. 2025, 9(9):1581883.
[31] Zhang Y, Xue C, Wang X, et al. The control effect of endophytic fungus Fusarium equiseti FUS-8 on cotton Verticillium wilt and its effects on soil microbial communities[J]. Plant and Soil, 2025, 512(1-2):1221-1241.
[32] 罗万珍,王丹,齐宏玥,等.拮抗细菌KRS022的鉴定及对大丽轮枝菌的抑制效果[J].中国农业科学, 2023, 56(4):649-664.
[33] 张春怡,曲红云,桑平,等.土壤消毒结合微生物菌剂防控茄子黄萎病及对根际真菌群落结构的影响[J].北方园艺, 2020(7):27-35.
[34] 詹林玉.嫁接茄子根际土壤微生物特性及黄萎病抗性研究[D].沈阳:沈阳农业大学, 2017.