Abstract: The effect of different carbon sources on the fermentation of Metarhizium anisopliae IMI330189 was determined in this paper. The effects of six carbon sources—glucose, sucrose, soluble starch, fructose, glucose＋ fluctose (1:1) and the starch hydrolysate on the growth and the metabolism of M. anisopliae were discussed. The results showed that glucose＋fluctose was the best carbon source among the six carbon sources, the biomass, growth rate and degradation time were 16.16 g/L, 0.4238 g/L·h, 48 h respectively, and the biomass were glucose＋fluctose＞sucrose＞glucose＞fructose＞soluble starch. The starch hydrolysate were found to be better for M. anisopliae, the biomass of M. anisopliae was improved 14.3% compared with soluble starch. So it was demonstrated that the utilization of polysaccharide was insufficient for M. anisopliae and the addition of exogenous enzyme might improve the fermentation level and decrease the cost of M. anisopliae.