Isolation and Identification of Plant Endophyte Bacterial Bacillus tequilens and Its Control Effect against Watermelon Fusarium Wilt

doi:10.16409/j.cnki.2095-039x.2017.03.011

Abstract

Abstract: For the purposes of selecting new biocontrol agents, bacteria wm031 was isolated from the tomato plants using the dilution plate method and its antifungal activity was tested by the dual plate assay. The strain wm031 was identified based on morphological characteristics, 16S rDNA sequence analysis, physiological and biochemical characteristics, and its antagonistic against plant diseases was investigated by greenhouse experiments. The results indicated that the strain wm031 exhibited broad-spectrum antagonism against the tested fungi, including Fusarium oxyporum f. sp. niveum, Sclerotinia sclerotiorum in oilseed rape, Bipolaris sorokinianum, Gaeumannomyces graminis var. tritici Walker, Phytophthora capsici, Sclerotinia allii Saw., Fusarium moniliforme Sheld, Colletotrichum gloeosporioides, and Fusarium oxysporum f. sp. lycopersici. The strain wm031 was identified as Bacillus tequilens, and it could stably colonize in watermelon and rice plants. Strain wm031 demonstrated good control effects on watermelon Fusarium wilt caused by Fusarium oxyporum f. sp. niveum, with the efficacy of 74.6%, higher than that of chlorothalonil by 14.1%. Thus, the strain wm031 is a potential biocontrol agent to be applied in the field.

Key words: Bacillus tequilens, isolation, biocontrol agents, Fusarium wilt of watermelon

CLC Number:

S476

ZHANG Meng, WANG Qiong, FENG Fayun, YUAN Jianjun, YU Xiangyang. Isolation and Identification of Plant Endophyte Bacterial Bacillus tequilens and Its Control Effect against Watermelon Fusarium Wilt[J]. journal1, 2017, 33(2/3): 371-377.

References

[1] Huang N, Enkegaard A, Osborne L S, et al. The banker plant method in biological control[J]. Critical Reviews in Plant Sciences, 2011, 30: 259-278.
[2] 周秀华, 崔磊, 武术杰, 等. 三种拮抗菌对苗木立枯病病原菌的影响[J]. 北方园艺, 2011(24): 167-169.
[3] Mbarga J B, Begoude B A D, Ambang Z, et al. A new oil-based formulation of Trichoderma asperellum for the biological control of cacao black pod disease caused by Phytophthora megakarya[J]. Biological Control, 2014, 77(1): 15-22.
[4] Kriaa M, Hammami I, Sahnoun M, et al. Biocontrol of tomato plant diseases caused by Fusarium solani using a new isolated Aspergillus tubingensis CTM 507 glucose oxidase[J]. Comptes Rendus Biologies, 2015, 338(10): 666-677.
[5] Ghosh R, Barman S, Khatun J, et al. Biological control of Alternaria alternata causing leaf spot disease of Aloe vera using two strains of rhizobacteria[J]. Biological Control, 2016, 97(1): 102-108.
[6] Tsuda K, Tsuji G, Higashiyama M, et al. Biological control of bacterial soft rot in Chinese cabbage by Lactobacillus plantarum strain BY under field conditions[J]. Biological Control, 2016, 100(1): 63-69.
[7] Sharifazizi M, Harighi B, Sadeghi A. Evaluation of biological control of Erwinia amylovora, causal agent of fire blight disease of pear by antagonistic bacteria[J]. Biological Control, 2017, 104(1): 28-34.
[8] 谢永丽, 徐志伟, 马莉贞, 等. 青海北山林场桦树根围芽胞杆菌分子鉴定及其拮抗活性分析[J]. 植物保护学报, 2012, 39(3): 246-252.
[9] 刘爽, 刘娟, 凌婉婷, 等. 一株高效降解菲的植物内生细菌筛选及其生长特性[J]. 中国环境科学, 2013, 33(1): 95-102.
[10] Nawangsih A A, Damayanti I K A, Wiyono S, et al. Selection and characterization of endophytic bacteria as biocontrol agents of tomato bacterial wilt disease[J]. HAYATI Journal of Biosciences, 2011, 18(2): 66-70.
[11] Ramesh R, Phadke G S. Rhizosphere and endophytic bacteria for the suppression of eggplant wilt caused by Ralstonia solanacearum[J]. Crop Protection, 2012, 37(1): 35-41.
[12] Aydi Ben Abdallah R, Mokni-Tlili S, Nefzi A, et al. Biocontrol of Fusarium wilt and growth promotion of tomato plants using endophytic bacteria isolated from Nicotiana glauca organs[J]. Biological Control, 2016, 97(1): 80-88.
[13] Ren J H, Li H, Wang Y F, et al. Biocontrol potential of an endophytic Bacillus pumilus JK-SX001 against poplar canker[J]. Biological Control, 2013, 67(3): 421-430.
[14] Yang R, Fan X, Cai X, et al. The inhibitory mechanisms by mixtures of two endophytic bacterial strains isolated from Ginkgo biloba against pepper phytophthora blight[J]. Biological Control, 2015, 85(1): 59-67.
[15] 胡桂萍, 郑雪芳, 尤民生, 等. 植物内生菌的研究进展[J]. 福建农业学报, 2010, 25(2): 226-234.
[16] 林英, 司春灿, 赵青松, 等. 一株死谷芽孢杆菌的分离、鉴定及抗病促生效果初探[J]. 北方园艺, 2014, 13: 88-92.
[17] 布坎南 R E, 吉本斯 N E. 伯杰细菌鉴定手册(第八版)[M]. 中国科学院微生物研究所译, 北京: 科学出版社, 1984.
[18] 南宇航, 朱子成, 王学征, 等. 甜瓜种质资源苗期对枯萎病和白粉病的抗性评价[J]. 中国蔬菜, 2016(1): 37-44.
[19] 李曹龙, 秦艳, 欧秀玲, 等. 高温产蛋白酶菌株的筛选及产酶条件的研究[J]. 中国酿造, 2012, 31(12): 98-101.
[20] 刘晓玲, 王金晶, 李永仙, 等. 产β-1,3-1,4-葡聚糖酶特基拉芽孢杆菌Bacillus tequilensis CGX5-1发酵培养基的优化[J]. 食品与生物技术学报, 2013, 32(6): 645-650.
[21] 王凯. 黄曲霉生防菌的筛选鉴定及高效菌株JPP1的生防机制[D]. 哈尔滨: 哈尔滨工业大学, 2013.
[22] 冯金龙. 马铃薯病原真菌拮抗性细菌鉴定及其生物学功能研究[D]．兰州: 甘肃农业大学, 2013.
[23] 谢洁, 任惠爽, 唐翠明, 等. 一株桑树内生细菌的鉴定和对桑椹核地杖菌的拮抗作用[J]. 蚕业科学, 2015, 41(5): 815-824.
[24] 蔡学清. 内生枯草芽孢杆菌BS-2(Bacillus subtilis)在植物体内的定殖及促生作用[D]. 福州: 福建农林大学, 2005.
[25] 高增贵, 庄敬华, 陈捷, 等. 应用免疫胶体金银染色技术定位玉米内生细菌[J]. 植物病理学报, 2005, 35(3): 262-266.
[26] 杨洪凤, 余向阳, 薛雅蓉, 等. 内生解淀粉芽孢杆菌CC09在小麦根部定殖的电镜观察及防病效果[J]. 中国生物防治学报, 2014, 30(6): 839-844.
[27] Ran L X, Liu C Y, Wu G J, et al. Suppression of bacterial wilt in Eucalyptus urophylla by fluorescent Pseudomonas spp. in China[J]. Biological Control, 2005, 32(1): 111-120.
[28] Simonetti E, Carmona M A, Scandiani M M, et al. Evaluation of indigenous bacterial strains for biocontrol of the frogeye leaf spot of soya bean caused by Cercospora sojina[J]. Letters in Applied Microbiology, 2012, 55(2): 170-173.
[29] Dal B G, Mónaco C, Rollan M C, et al. Biocontrol of postharvest grey mould on tomato by yeasts[J]. Journal of Phytopathology, 2008, 156(5): 257-263.