Screening of Biocontrol Streptomyces against Fusarium Wilt of Watermelon and Its Study on Control Mechanism

doi:10.16409/j.cnki.2095-039x.2023.02.032

Abstract

Abstract: In order to prevent and control watermelon Fusarium wilt safely and efficiently, in this study, seven Streptomyces with good antagonistic effect against Fusarium oxysporum f. sp. niveum (FON) were selected using plate confrontation method. The inhibitory effect of Streptomyces fermentation filtrate and bacterial crude extract on the growth of FON was investigated. Among these seven Streptomyces, Streptomyces tunisialbus YC2-17 and S. eurocidicus YC5-15 showed strong inhibitory effect on FON,with inhibitory rates of 56.21% and 54.72%. In addition, the plant growth-promoting experiments on watermelon seedlings were carried out. The results showed that the two Streptomyces had significant promoting effects on the growth of watermelon seedlings, and YC2-17 had better promoting effect. After YC2-17 treatment, the plant height, root length, fresh weight and dry weight of watermelon seedlings increased by 52.54%, 27.65%, 109.57% and 100.00%. The result of the potted plant experiments demonstrated that the control effects of YC2-17 and YC5-15 fermentation solution on watermelon Fusarium wilt were 56.82% and 50.45%, respectively. The treatment of ethyl acetate phase crude extract of YC2-17 fermentation filtrate could inhibit the formation and germination of FON spores, change the morphology of spores and mycelium. After treatment, the external morphology of hyphae and spores was abnormal, the trunk were flat, and the contents were exuded.

Key words: biocontrol, Fusarium wilt of watermelon, Streptomyces, antifungal substance

CLC Number:

S436.5

TIAN Lin, CHEN Jing, WANG Yang. Screening of Biocontrol Streptomyces against Fusarium Wilt of Watermelon and Its Study on Control Mechanism[J]. Chinese Journal of Biological Control, 2023, 39(3): 657-666.

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