Screening and Effect of Antagonistic Actinomycetes against Anthracnose of Cymbidium ensifolium

doi:10.16409/j.cnki.2095-039x.2019.05.029

Abstract

Abstract: In order to screen the actinomycetes with good antifungal effect against the anthracnose pathogen on Cymbidium ensifolium, actinomycetes were isolated from surface soil sample of wild mountain orchid. The results showed that a total of 25 strains of actinomycetes were isolated, and 6 strains had obvious inhibitory effect on Colletotrichum gloeosporioides of C. ensifolium. Strain SVFJ-07 had the best effect with the inhibitory rate of 83.64%. Based on morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis, the strain SVFJ-07 was identified as Streptomyces vinaceus. It had good inhibition effect on several fungi. The sterile fermentation broth could inhibit C. ensifolium of the anthracnose by 70.06% and 66.27%-68.54% in the lab and in the field, respectively. All the results indicated that strain SVFJ-07 was effective to control C. gloeosporioides of C. ensifolium, and had promising application prospect in biological control.

Key words: Cymbidium ensifolium, anthracnose, actinomycete, antifungal effect, screening and identification

CLC Number:

S436.8
S476

YAO Jinai, HUANG Peng, CHEN Feng, HUANG Minmin, YU Deyi. Screening and Effect of Antagonistic Actinomycetes against Anthracnose of Cymbidium ensifolium[J]. Chinese Journal Of Biological Control, 2019, 35(5): 805-812.

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