Compound Mutation of Brevibacillus brevis Strain Bb5911 and Its Control Effect on Banana Anthracnose

doi:10.16409/j.cnki.2095-039x.2016.06.014

Abstract

Abstract: In this study, the antagonist Brevibacillus brevis strain Bb5911 was mutated by UV-NaNO₂ compound in order to improve the antifungal activity against banana anthracnose. In addition, the control effects of the mixture of this strain and prochloraz were tested for investigating its feasibility mixed with fungicide. The results showed that, the optimum mutation condition was irradiated 15 s under 20 W UV lamps at the distance of 50 cm, and then treated 60 min with 300 mmol/L NaNO₂. After twice mutations, the strongest antifungal strain 5B37-3 was selected out. Its antifungal activity was 112.41% higher than that of the original strain. The minimal inhibitory concentration (MIC) of prochloraz against strain 5B37-3 was 20.83 μg/mL. The antifungal activity of strain 5B37-3 was not reduced significantly after transferring 7 times continuously. The results of inhibition spectrum test showed that the antifungal activity of strain 5B37-3 against 13 plant pathogens were all higher than that of the original strain, with the increase rate from 18.20% to 239.27%. Furthermore, the control efficiency of strain 5B37-3 on banana anthracnose was 66.55%, which was significantly higher than that of the original strain. After 15 days storage, the control efficiency of the mixture of strain 5B37-3 and 10.42 μg/mL prochloraz was 72.76%, which was similar to the 333.33 μg/mL prochloraz. But after 17 days storage, it showed lower than that of 333.33 μg/mL prochloraz significantly.

Key words: Brevibacillus brevis, compound mutation, banana anthracnose, control effect

CLC Number:

S476

DU Chanjuan, FU Gang, PAN Lianfu, YE Yunfeng, ZHANG Jin, YANG Di, SUN Jian. Compound Mutation of Brevibacillus brevis Strain Bb5911 and Its Control Effect on Banana Anthracnose[J]. journal1, 2016, 32(6): 767-774.

References

[1] Korsten L. Advances in control of postharvest diseases in tropical fresh produce[J]. International Journal of Postharvest Technology and Innovation, 2006, 1(1):48-61.
[2] Peeran M F, Krishnan N, Thangamani P R, et al. Development and evaluation of water-in-oil formulation of Pseudomonas fluorescens (FP7) against Colletotrichum musae incitant of anthracnose disease in banana[J]. European Journal of Plant Pathology, 2014, 138(1):167-180.
[3] 李静, 冯淑杰, 肖晶, 等. 小白菜内生假单胞菌XBC-PS的生防作用[J]. 中国蔬菜, 2007, (5):21-23.
[4] de Costa D M, Erabadupitiya H R U T. An integrated method to control postharvest diseases of banana using a member of the Burkholderia cepacia complex[J]. Postharvest Biology and Technology, 2005, 36(1):31-39.
[5] Lassois L, de Bellaire L D L, Jijakli M H. Biological control of crown rot of bananas with Pichia anomala strain K and Candida oleophila strain O[J]. Biological Control, 2008, 45(3):410-418.
[6] Alvindia D G, Natsuaki K T. Evaluation of fungal epiphytes isolated from banana fruit surfaces for biocontrol of banana crown rot disease[J]. Crop Protection, 2008, 27(8):1200-1207.
[7] 李振华, 凌金锋, 曾会才. 6株土壤链霉菌的抑真菌活性及其发酵液对采后香蕉果实炭疽病的防效[J]. 热带农业科学, 2006, 26(3):35-37, 55.
[8] 谭志琼, 张荣意, 杨劲松, 等. 海洋青霉T03对4种热带植物病原真菌的拮抗作用[J]. 中国生物防治, 2006, 22(s):112-114.
[9] 刘兴荔, 连云鹏. 放线菌618菌株发酵液防病应用试验[J]. 生物防治通报, 1987, 3(2):50-54.
[10] 何红, 蔡学清, 陈玉森, 等. 辣椒内生枯草芽孢杆菌BS-2和BS-1防治香蕉炭疽病[J]. 福建农林大学学报(自然科学版), 2002, 31(4):442-444.
[11] 陈弟, 殷晓敏, 张荣意. 内生枯草芽孢杆菌B215对香蕉炭疽菌抑制作用初探[J]. 广西热带农业, 2008, (1):1-2.
[12] 李敏, 胡美姣, 高兆银, 等. 香蕉炭疽病拮抗菌C71菌株的诱变选育[J]. 热带农业科学, 2008, 28(6):6-10.
[13] Alvindia D G, Natsuaki K T. Biocontrol activities of Bacillus amyloliquefaciens DGA14 isolated from banana fruit surface against banana crown rot-causing pathogens[J]. Crop Protection, 2009, 28(3):236-242.
[14] Fu G, Huang S L, Ye Y F, et al. Characterization of a bacterial biocontrol strain B106 and its efficacy in controlling banana leaf spot and post-harvest anthracnose diseases[J]. Biological Control, 2010, 55(1):1-10.
[15] 中国人民共和国国家质量监督检验检疫总局[S]. GB/T 17980.96-2004农药田间药效试验准则(二)第96部分:杀菌剂防治香蕉贮藏病害.
[16] 付岗, 叶云峰, 吴永官, 等. 7株拮抗菌的鉴定及其对香蕉炭疽病的防治作用[J]. 热带作物学报, 2015, 36(2):358-364.
[17] Edwards S G, Seddon B. Brevibacillus brevis as a biocontrol agent against Botrytis cinerea on protected Chinese cabbage//Recent Advances in Botrytis Research. Proceedings of the 10th International Botrytis Symposium[C]. Backhuys Publisher, 1992, 267-271.
[18] 汪茜, 胡春锦, 柯仿钢, 等. 生防菌株1404的鉴定及其对采后柑橘炭疽病的防治效果[J]. 微生物学报, 2010, 50(9):1208-1217.
[19] 易有金, 尹华群, 罗宽, 等. 烟草内生短短芽孢杆菌的分离鉴定及对烟草青枯病的防效[J]. 植物病理学报, 2007, 37(3):301-306.
[20] 车建美, 刘波, 蓝江林. 短短芽孢杆菌JK-2的GFP标记及其抑菌作用[J]. 中国生物防治, 2010, 26(2):230-234.
[21] 陈莉, 缪卫国, 刘海洋, 等. 短短芽孢杆菌A57对棉花主要病原真菌的拮抗作用及其最佳液体发酵条件[J]. 中国生物防治, 2007, 23(s):22-27.
[22] Hwang J Y, Shim C K, Ryu K Y. Selection of Brevibacillus brevis B23 and Bacillus stearothermphilus B42 as biological control agents against sclerotinia rot of lettuce[J]. Research in Plant Disease, 2006, 12(3):254-259.
[23] Haggag W M. Isolation of bioactive antibiotic peptides from Bacillus brevis and Bacillus polymyxa against Botrytis grey mould in strawberry[J]. Archives of Phytopathology and Plant Protection, 2008, 41(7):477-491.
[24] Sunita C, Eunice J A, Steve W. Biological control of Fusarium osysporum f. sp. lycopersici on tomato by Brevibacillus brevis[J]. Journal of Phytopathology, 2010, 158(7-8):470-478.
[25] Walker R, Powell A A, Seddon B. Brevibaeillus isolates from the spermosphere of peas and dwarf French beans with antifungal activity against Botrytis cinerea and Pythium species[J]. Journal of Applied Microbiology, 1998, 84(5):791-801.
[26] 张璐, 丁延芹, 杜秉海, 等. 黄瓜枯萎病病原拮抗细菌DS-1菌株鉴定及其生防效果研究[J]. 园艺学报, 2010, 37(4):575-580.
[27] 杨廷雅, 孙亮, 周婷婷, 等. 短短芽孢杆菌Brevibacillus brevs HAB-5主要抑菌活性成分的分析及其特性研究[J]. 中国生物防治学报, 2014, 30(2):222-231.
[28] 付岗, 叶云峰, 吴永官, 等. 香蕉采后炭疽病生防菌的多重筛选研究[J]. 云南农业大学学报, 2015, 30(4):554-559.
[29] 谭志琼, 淦国英, 张荣意. 枯草芽孢杆菌B68对香蕉果实潜伏炭疽菌的抑制作用[J]. 广西热带农业, 2006, (5):1-4.
[30] 柳凤, 陈振明, 何红. CⅢ-1菌株胞外抗菌蛋白防治香蕉炭疽病研究[J]. 果树学报, 2010, 27(4):580-584.