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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (1): 65-71.DOI: 10.16409/j.cnki.2095-039x.2019.05.023

• RESEARCH REPORTS • Previous Articles     Next Articles

Identification of Synomones Emitted from Tea Plants Damaged by Ectropis grisescens

FAN Peizhen1,2, HAN Shanjie1, HAN Baoyu1   

  1. 1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine/College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
    2. Liu'an Vocational Technical College, Liu'an 237158, China
  • Received:2019-05-24 Online:2020-02-08 Published:2020-02-26

Abstract: In order to identify the synomones released from tea plants damaged by Ectropis grisescens, the volatile compounds from E. grisescens-damaged tea shoots (EGDTS) and the intact tea shoots (ITS) were collected by the simultaneous distillation extraction method (SDE), and then detected by gas chromatography coupled of mass spectrometer. Thirty-one and twenty-five components were respectively identified, and the total contents of these volatile components from the two samples relative to internal standards of the same quality were respectively 1863.8% and 1124.6%. Compared with the volatiles from ITS, ample benzaldehyde, E-2-decenal and indole were detected in volatiles from EGDTS and contents of salicylic acid, E-2-hexenal and hexanal were significantly higher. The eighteen principal components identified from EGDTS were respectively dissolved in liquid paraffin to make a series of odor sources at 10-4g/mL, and responses of the parasitic wasp Apanteles sp. to these odors were observed using Y-tube olfactometer. The results showed that benzaldehyde, compared with liquid paraffin, benzaldehyde, salicylic acid, E-2-hexenal and hexanal significantly attracted the wasps, E-2-decenal, 4-heptenal and indole showed obvious attraction, while the other eleven components showed no attraction. The seven components showing attractive activities were further prepared as attractants at 10-2g/mL and then loaded onto rubber septa. A rubber spetum was singly attached on a bud green sticky board and used to trap the parasitic wasp at its peak occurrence. The wasp catches of the former 4 components were obviously more than those of the latter 3 components, and significantly more than those of the blank control. Especially, the blend of equal amount of benzaldehyde, salicylic acid and E-2-hexenal at 10-2g/mL showed the strongest attraction. Therefore, benzaldehyde, salicylic acid, E-2-hexenal and hexanal are the synomones of tea plant induced by E. grisescens.

Key words: Ectropis grisescens, synomone of tea plant, attraction, Apanteles sp., lure

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