Isolation, Identification and Insecticidal Activity of Pathogenic Serratia marcescens from Potato Tuber Moth Larvae

doi:10.16409/j.cnki.2095-039x.2020.03.003

Abstract

Abstract: A strain ML-1 showing strong pathogenicity to larvae of potato tuber moth was isolated from the natural diseased larvae of potato tuber moth collected from potato fields. The strain ML-1 was identified as S. marcescens by morphological observation of pathogens and 16S rRNA sequencing analysis. The virulence of the strain ML-1 suspension, fermentation broth and fermentation supernatant to the 3-d-old larvae of potato tuber moth was determined by feeding method. The cumulative corrected mortalities were 63.16%, 80.70% and 49.12% on the 7th day after feeding, respectively, and the LT₅₀ were 3.04, 4.71 and 6.47 d at 3.0×10⁹ cfu/mL, respectively. The LC₅₀ at the 3rd, 5th and 7th day after treatment with fermentation broth were 9.784x10¹⁰, 1.855x10⁸ and 5.434x10⁵ cfu/mL, respectively. In summary, S. marcescens ML-1 strain has strong virulence to potato tuber moth larvae and has good prospective for development of biological control agents against the pest.

Key words: potato tuber moth, Serratia marcescens, virulence determination, pathogenicity, insecticidal activity

CLC Number:

S476

SU Zaotang, ZHANG Lingying, XU Tianmei, DU Guangzu, CHEN Bin, XIAO Guanli. Isolation, Identification and Insecticidal Activity of Pathogenic Serratia marcescens from Potato Tuber Moth Larvae[J]. Chinese Journal Of Biological Control, 2020, 36(3): 361-370.

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