Chimecal Composition, Insecticidal and Repellent Activities of Essential Oils from Two Chamaecyparis Species against Mosquitoes

doi:10.16409/j.cnki.2095-039x.2018.03.002

Abstract

Abstract: The insecticidal potential of the essential oils from Chamaecyparis pisifera and Chamaecyparis obtuse were tested against the 4th instar larvae and pupae of Aedes albopictus and Culex pipiens quinquefasciatus using the immersion method. The repellent activity of the essential oils against adults of A. albopictus was assayed by topical application on the skin. The compositions of essential oils were qualitatively analyzed using gas chromatography-mass spectrometry. The 24 h LC₅₀ values of C. pisifera and C. obtuse oil against the 4^th instar larvae/pupae of A. albopictus were 47.66/152.33 and 73.11/205.06 μg/mL, respectively, while for C. p. quinquefasciatus the values were 31.74/107.61 and 63.94/131.42 μg/mL, respectively. The protection period offered by C. pisifera and C. obtuse oil against A. albopictus were 3.24 h and 0.78 h, 5.45 h and 1.86 h at concentration of 1.5, 2.0 μL/cm², respectively. A total of 16 compounds were identified from C. pisifera oil, including α-pinene (29.89%), 17-norkaur-15-ene (9.96%), Δ-3-carene (9.79%). And 22 compounds were identified in C. obtuse oil, the main components were Δ-3-carene (35.33%), α-pinene (25.60%), β-myrcene (12.86%). The essential oils of C. pisifera and C. obtuse showed high larvicidal and pupicidal activity against the 4^th instar larvae and pupae of A. albopictus and C. p. quinquefasciatus, and C. pisifera oil also had excellent repellent activity against A. albopictus. The results suggest that C. pisifera oil and C. obtuse oil could be exploited as potential eco-friendly mosquito control agents.

Key words: Chamaecyparis pisifera, Chamaecyparis obtuse, essential oil, mosquitoes, insecticidal activity, repellent activity

CLC Number:

S482.3

PENG Yinghui, ZHAO Helu, XIONG Guohong, FU Qiaomei, LIU Min, JIANG Lijuan, LI Jilie. Chimecal Composition, Insecticidal and Repellent Activities of Essential Oils from Two Chamaecyparis Species against Mosquitoes[J]. journal1, 2018, 34(3): 364-369.

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