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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (2): 297-303.DOI: 10.16409/j.cnki.2095-039x.2021.02.019

• RESEARCH REPORTS • Previous Articles    

Isolation and Identification of Hyperparasitic Fungi in Phyllactinia moricola on Mulberry

HAN Ningning, WANG Zhengxian, HUANG Yiman, LIAO Yongmei   

  1. College of Agriculture, Guangxi University, Nanning 530005, China
  • Received:2020-02-27 Published:2021-04-14

Abstract: To understand the species of hyperparasitic fungi of Phyllactinia moricola, fungal isolation from P. moricola and hyperparasitism verification were conducted, and two hyperparasitic fungal strains HP8 and HP9 were obtained. In cleistothecia of P. moricola parasitized by strains HP8 or HP9, the asci and ascospores were destroyed. The colonies of strain HP8 were green in color, the mycelia were brown or colorless, with septa, conidiophores with nodular swellings on the septa, conidia oval, unicellular, brown or colorless. ITS sequence (MT463536) had 100% identity to that in Cladosporium oxysporum (MF135506). In phylogenetic tree, ITS sequence of strain HP8 clustered together with C. oxysporum (MF135506). The colonies of HP9 strain were white in color, the mycelia were colorless, conidia crescent, unicellular and colorless. ITS sequence (MT463537) had 99% identity to that in Lecanicillium psalliotae (KC881072). In phylogenetic tree, ITS sequence of strain HP9 clustered together with L. psalliotae (MT463537). Combined with morphological characteristics and ITS sequence analysis results, strains HP8 and HP9 were identified as C. oxysporum and L. psalliotae, respectively. The results of high-throughput sequencing showed that L. psalliotae was the main hyperparasitic fungal species on P. moricola in diseased mulberry leaves.

Key words: Phyllactinia moricola, hyperparasitic fungus, Cladosporium oxysporum, Lecanicillium psalliotae

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