Physical and Chemical Properties of Antimicrobial from Bacillus amyloliquefaciens Strain MH71 and Its Inhibition Activities against Botrytis cinerea

doi:10.16409/j.cnki.2095-039x.2016.04.010

Abstract

Abstract: MH71 is a strain with a broad-spectrum of antagonistic activities, and this research was conducted to characterize physical and chemical properties and antimicrobial activity of the active substances produced by the strain. The stability of the antimicrobial substances were studied, and their activities against Botrytis cinerea were tested. By acid precipitation and ammonium sulfate precipitation, lipopeptide and protein active substances were obtained, and these two active substances were then detected by HPLC and SDS-PAGE. Incubated at 100 ℃ for 60 min and 121 ℃ 30 min, the relative antimicrobial activity of the substances is 88.98% and 79.60%, respectively, and incubated at pH 3-11, the relative antimicrobial activity still remained very high, and moreover, the acitvties were insensitive to UV, protease K, pepsin and trypsin. These results suggested that the antimicrobial substances produced by MH71 had a strong heat resistance and pH adaptability. When the mycelium of B. cinerea was treated by different concentrations of the MH71 fermentation broth, lots of abnormal hyphae were observed such as swelling or overflow. Compared with the antimicrobial peptides, the lipopeptides showed stronger activities to inhibit B. cinerea. One protein band with a molecular of 40 kD was detected by SDS-PAGE, and HPLC test showed that surfactin existed in the antimicrobial substances produced by MH71.

Key words: Bacillus amyloliquefaciens, antimicrobial substances, physical and chemical properties, Botrytis cinerea, antagonistic activity

CLC Number:

S476
S432.1

LI Hongxiao, ZHANG Dianpeng, ZHAO Hongxin, LIU Weicheng, LU Caige. Physical and Chemical Properties of Antimicrobial from Bacillus amyloliquefaciens Strain MH71 and Its Inhibition Activities against Botrytis cinerea[J]. journal1, 2016, 32(4): 485-492.

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