Welcome to Chinese Journal of Biological Control,Today is

Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (3): 480-485.DOI: 10.16409/j.cnki.2095-039x.2021.01.017

• RESEARCH REPORTS • Previous Articles    

Identification and Biological Characteristics of Entomopathogenic Nematode JLSY 003

TIAN Chengli, LI Maohai, LIU Jinwen, ZHANG Jinhua, ZHU Feng, LI Jianping   

  1. Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100
  • Received:2019-05-28 Published:2021-06-16

Abstract: The entomopathogenic nematode was isolated from the soil samples collected in Songyuan, Jilin province, China. It was identified as Oscheius myriophila strain by ITS and 28S rDNA sequences, and named JLSY003. Last instar of Galleria mellonella was used as host to test JLSY003 biological characteristics. The results showed that it had a high pathogenicity at 80 IJs/larva, LT50 was 15.409 h. LC50 was 46.067 and 25.297 IJs/larva at 24 and 48 h, respectively. JLSY 003 had a high temperature tolerance and a high fertility. The nematode mortality rate was 19.27% at 36℃ and for 8 h, the yield was 1.873×105 IJs/larva or 4.695×105 IJs/g. The results showed that JLSY003 had a high pathogenicity, a high temperature torlerance and a high fertility.

Key words: entomopathogenic nematodes, Oscheius myriophila, LC50, LT50, high temperature tolerance

CLC Number: