Synergistic Control of Trichoderma longibrachiatum and Bacillus velezensis against Sugarcane Red Rot

doi:10.16409/j.cnki.2095-039x.2026.02.001

Abstract

Abstract: In order to clarify the synergistic control effect of Trichoderma longibrachiatum SOR-1 and Bacillus velezensis YC89 against sugarcane red rot, explored the sequence of strain addition and fermentation time in the co-culture system by.using mycelial growth rate method. Then, pot experiments were conducted to evaluate the control efficacy of the co-culture fermentation broth (COYS) against sugarcane red rot and its impact on the activity of defense-related enzymes in leaves. The results showed that the treatment sequence of first inoculating YC89 followed by SOR-1 and co-culturing for 5 days exhibited the strongest inhibitory effect against Fusarium oxysporum. The control effect of COYS on sugarcane red rot is the best, at 62.25%, which is 20.41%, 10.21%, and 2.05% higher than the control effects of SOR-1 and YC89 single culture fermentation broth and compound fermentation broth YC89+SOR-1, respectively. Additionally, the activities of POD, CAT, PAL, and SOD in sugarcane leaves treated with COYS were significantly higher than those in the control group (CK), with peak values being 3.02, 5.45, 3.63 and 2.60 times that of CK, respectively. In conclusion, the co-cultured fermentation both of SOR-1 and YC89 demonstrated superior disease control efficacy compared to single-cultured broths, which provides a theoretical basis for the synergistic biological control of sugarcane red rot disease.

Key words: sugarcane red rot disease, Trichoderma longibrachiatumlong, Bacillus velezensis, co-culture, Collaborative prevention and control

CLC Number:

S476

DONG Lingling, ZHANG Jianping, HE Difei, LI Xianli, CAO Hongyu, LI Yaming, HE Lilian, LI Fusheng. Synergistic Control of Trichoderma longibrachiatum and Bacillus velezensis against Sugarcane Red Rot[J]. Chinese Journal of Biological Control, 2026, 42(1): 55-63.

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