黄花蒿叶醇提取物除草活性物质的分离及结构鉴定

doi:10.16409/j.cnki.2095-039x.2021.03.004

摘要/Abstract

摘要： 为明确黄花蒿除草活性及活性物质的成分，本研究采用室内生测法测定了黄花蒿叶醇提物对4种杂草的活性；采用系统溶剂分离、硅胶柱层析分离及气质联用色谱法对提取物进行了分离和结构鉴定。结果表明：5.0 mg/mL的黄花蒿叶醇提物对供试杂草发芽率、发芽势和发芽指数均有一定的抑制作用，抑制作用从大到小的顺序为反枝苋，苘麻，狗尾草，稗，其中对反枝苋、苘麻和狗尾草发芽率的抑制作用较强，均达到85%以上。叶醇提物对杂草发芽势的抑制作用也很强，均达到73%以上。黄花蒿叶醇提物中共鉴定出17种化合物，主要包括青蒿酸、亚油酸和邻苯二甲酸二乙酯等。由此可见，黄花蒿叶醇提物含有大量的除草活性物质，可显著延缓杂草的发芽时间和减少杂草的发芽率。

关键词: 黄花蒿, 除草活性, 分离, 鉴定

Abstract: The herbicidal activity and active components of Artemisia annua Linn. were determined by laboratory bioassay of herbicidal activity of ethanolic extract from Artemisia annua leaves against four weeds (Amaranthus retroflexus L., Abutilon theophrasti Medic., Setaria viirdis L. Beauv. and Echinachlao crusgali L. Beauv.) and by separation and identification of the extract using solvent separation, silica gel column chromatography and gas chromatography-mass spectrometry (GC-MS). The results showed that, at a concentration of 5.0 mg/mL, the ethanolic extract from the leaves of A. annua showed certain inhibitory effects on the germination rate, germination potential and germination index of weeds. The order inhibitory effects from high to low was redroot pigweed, velvetleaf, green bristlegrass and barnyard grass. Germination was inhibited at above 85% in redroot pigweed, velvetleaf, green bristlegrass and barnyard grass. Germination potential of redroot pigweed, velvetleaf, green bristlegrass and barnyard grass was also strongly inhibited, all at above 73.36%. A total of 17 compounds were identified from the ethanolic extract of A. annua leaves, mainly including gartemisinic acid, (Z,Z)-9,12-octadecadienoic acid, and diethyl phthalate, among the others. Therefore, there are a lot of herbicidal active substances in ethanolic extract of A. annua leaves, which can significantly delay the germination and reduce the germination rate of weeds.

Key words: Artemisia annua, herbicidal activity, separation, identification

中图分类号:

S476

滕春红, 冯曦茹, 徐永清, 谭洪鹤, 马艺倩, 陶波. 黄花蒿叶醇提取物除草活性物质的分离及结构鉴定[J]. 中国生物防治学报, 2021, 37(2): 244-250.

TENG Chunhong, FENG Xiru, XU Yongqing, TAN Honghe, MA Yiqian, TAO Bo. Isolation and Identification of Herbicidal Substance from Ethanolic Extracts of Artemisia annua Linn Leaf[J]. Chinese Journal of Biological Control, 2021, 37(2): 244-250.

参考文献

[1] Rowen D J, Templeman M A, Kingsford M J. Herbicide effects on the growth and photosynthetic efficiency of Cassiopea maremetens[J]. Chemosphere, 2017, 29(5):1-12.
[2] Owagboriaye F O, Dedeke G A, Ademolu K O, et al. Reproductive toxicity of roundup herbicide exposure in male albino rat[J]. Experimental and Toxicologic Pathology, 2017, 69(7):461-468.
[3] 闫超, 陈敏, 周颖, 等. 石蝉草乙醇提取物除草活性初探[J]. 植物保护, 2018, 44(2):199-203.
[4] 强胜, 陈世国. 生物除草剂研发现状及其面临的机遇与挑战[J]. 杂草科学, 2011, 29(1):1-6.
[5] Dayan F E, Owens D K, Watson S B, et al. Sarmentine, a natural herbicide from Piper species with multiple herbicide mechanisms of action[J]. Frontiers in Plant Science, 2015, 6:222.
[6] Grossmann K, Hutzler J, Tresch S, et al. On the mode of action of the herbicides cinmethylin and 5-benzyloxym-ethyl-1, 2-isoxazolines:putative inhibitors of plant tyrosine aminotransferase[J]. Pest Management Science, 2012, 68(3):482-492.
[7] Dayan F E, Cantrell C L, Duke S O. Natural productsin crop protection[J]. Bioorganic &Medicinal Chemistry, 2009, 17(12):4022-4034.
[8] 陈世国, 强胜. 生物除草剂研究与开发的现状及未来的发展趋势[J]. 中国生物防治学报, 2015, 31(5):770-779.
[9] 滕春红, 陶波, 吕志超, 等. 植物源除草剂研究进展[J]. 农药, 2013, 52(9):632-634.
[10] 沈慧敏, 郭鸿儒, 黄高宝. 不同植物对小麦、黄瓜和萝卜幼苗化感作用潜力的初步评价[J]. 应用生态学报, 2005, 16(4):740-743.
[11] 赵红梅. 黄花蒿(Artemisia annua L.)化感物质的释放途径及其主要化感物质的分离鉴定[D]. 兰州:甘肃农业大学, 2008.
[12] 滕春红, 刘永双, 崔书芳, 等. 猪毛蒿除草活性物质的超声辅助提取工艺研究[J]. 现代化农业, 2013(7):66-68.
[13] 陈业兵, 王金信, 吴小虎, 等. 银胶菊的花对稗草的化感作用及其化感物质分离与鉴定[J]. 植物保护学报, 2010, 37(1):74-77.
[14] 卢海博, 万树青. 黄皮甲醇提取物的除草活性及有效成分研究[J]. 农药, 2012, 51(7):539-542.
[15] 邹晓锦, 孙占祥, 于涛, 等. 仁用杏叶片提取物对8种杂草种子萌发和幼苗生长的化感效果[J]. 农药, 2013, 52(6):454-456.
[16] 陈晓平. 食品理化检验[M]. 北京:中国计量出版社, 2008.
[17] 汪茂田, 谢培山, 王忠东. 天然有机化合物提取分离与结构鉴定[M]. 北京:化学工业出版社, 2004, 9.
[18] 刘志明, 王海英, 刘姗姗, 等. 小蓬草精油活性组分分析[J]. 生物质化学工程, 2010, 44(1):22-26.
[19] 杨超, 张海静, 杨旭, 等. GC-O与GC-MS联用法鉴定香竹竹叶中关键气味活性物质[J]. 竹子研究会刊, 2009, 28(4):40-44.
[20] 高兴祥, 李美, 房锋, 等. 小飞蓬水浸提液对杂草萌发和生长的抑制效果[J]. 草业科学, 2015, 32(1):48-53.
[21] 孙文浩, 吴厚铭. 凤眼莲根系分泌物中的克藻化合物[J]. 植物生理学报, 1993, 19(1):92-96.
[22] 张城嘉, 李祖任, 柏连阳. 棉籽壳中除草活性物质提取及鉴定[J]. 农药学学报, 2019, 21(2):146-150.
[23] Putnamet A R, Nair M G, Barnes R R, et al. Allelopathy:aviable weed contral stragegr[J]. Weed Technology, 1900, 1:20-27.
[24] 黄翔杰, 李宇超, 蒋鹏, 等. 野菊根系分泌物的鉴定及其对三种植物的化感效应[J]. 湖北农业科学, 2017, 56(6):1061-1065, 1071.
[25] 李文瑾. 葡萄(Vitis vinifera L.)化感物质的鉴定及化感作用研究[D]. 兰州:甘肃农业大学, 2010.
[26] Elakovich S D, Wooten J W. Allelopathic, Herbaceous, Vascular Hydrophytes[J]. Acs Symposium, 1995, 58-73.
[27] 王小兵, 路永明, 刘五星, 等. 花生根分泌物的鉴定及其化感作用[J]. 生态学杂志, 2011, 30(12):2803-2808.
[28] Chon S U, Choib S K, Jung S, et al. Effects of alfalfa leaf extracts and phenolic allelochemicals on early seedling growth and root morphology of alfalfa and barnyard grass[J]. Crop Protection, 2002(21):1077-1082.
[29] Huang L., Liu J.F., Liu L.X. Studies on the anti-pyretie and anti-inflammatory effects of Artemisia annual L.[J]. Journal Chinese Marteria medica, 1993, 18:44-48.
[30] Dhingra V, Pakki S R, Narasu M L. Antimicrobial activity of artemisinin and its preeursors[J]. Current Science, 2000, 78:708-710.
[31] 陈芝兰. 凤眼莲根对赤潮藻生长的抑制作用及其机理研究[D]. 广州:暨南大学, 2005.