贝莱斯芽胞杆菌ZZBV-3的鉴定及其对草莓根腐病的防效

doi:10.16409/j.cnki.2095-039x.2021.02.005

摘要/Abstract

摘要： 为获得草莓根腐病原菌尖孢镰刀菌Fusarium oxysporum的优良拮抗菌，以草莓种植基地的土壤为拮抗菌来源，通过室内测定不同菌株的抑菌活性，筛选抑菌率最高的菌株，在此基础上对所筛菌株进行分类鉴定，并评价其抑菌谱和防效。结果表明，从土壤中分离获得39株拮抗菌，筛选出3株对尖孢镰刀菌具有明显抑制作用的菌株。其中，菌株ZZBV-3的抑菌作用最强，抑菌圈直径为16.2 mm。经形态学及多基因（包括16s rDNA和gyrB基因）比对分析，确认菌株ZZBV-3为贝莱斯芽胞杆菌Bacillus velezensis。菌株ZZBV-3抑菌谱广，对多种植物病原菌均具有良好的抑菌效果。盆栽试验结果表明，菌株ZZBV-3对草莓“红颜”和“甜查理”品种的根腐病防效分别为77.96%和81.18%，生防应用前景良好。

关键词: 贝莱斯芽胞杆菌, 筛选鉴定, 草莓根腐病, 尖孢镰刀菌, 防治效果

Abstract: In order to obtain excellent antagonistic bacteria against Fusarium oxysporum, a pathogen causes strawberry root rot, the soil from strawberry planting base was taken as antagonistic bacteria source, and the strains with the highest bacteriostatic rate were screened by indoor determination of their bacteriostatic activities. Then, the screened strains were classified and identified, and their bacteriostatic spectrum and control efficacy were evaluated. The results showed that total 39 strains were isolated from soil, among which 3 strains with strong inhibitory efficacy on F. oxysporum were obtained. Among them, strain ZZBV-3 had the strongest antibacterial activity, and its antibacterial diameter was up to 16.2 mm. According to morphological, physiological and polygenic (including 16S rDNA and gyrB genes) analyses, strain ZZBV-3 was identified as Bacillus velezensis. Strain ZZBV-3 had a wide antibacterial spectrum and has good antibacterial effect on various plant pathogens. The results of pots experiment showed that the control efficacy of strain ZZBV-3 on root rot of strawberry varieties ‘Hongyan’ and ‘Sweet Charlie’ were 77.96% and 81.18%, respectively, and the application prospect of biocontrol was acceptable.

Key words: Bacillus velezensis, screening and identification, strawberry root rot, Fusarium oxysporum, control efficacy

中图分类号:

姚锦爱, 黄鹏, 赖宝春, 余德亿. 贝莱斯芽胞杆菌ZZBV-3的鉴定及其对草莓根腐病的防效[J]. 中国生物防治学报, 2021, 37(1): 172-177.

YAO Jinai, HUANG Peng, LAI Baochun, YU Deyi. Identification and Control Efficacy of Bacillus velezensis ZZBV-3 against Strawberry Root Rot[J]. Chinese Journal of Biological Control, 2021, 37(1): 172-177.

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