小菜蛾Btk抗性品系对四种Bt杀虫晶体蛋白抗性发展的研究

doi:10.16409/j.cnki.2095-039x.2021.05.019

中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (2): 328-332.DOI: 10.16409/j.cnki.2095-039x.2021.05.019

小菜蛾Btk抗性品系对四种Bt杀虫晶体蛋白抗性发展的研究

符伟¹, 唐涛¹, 王培¹, 王勇², 尹丽², 马明勇¹, 张政兵²

1. 湖南省农业科学院植物保护研究所，长沙 410125；2.湖南省植保植检站，长沙410005

收稿日期:2020-12-17 出版日期:2022-04-08 发布日期:2022-04-20
通讯作者: 符伟;张政兵,硕士,农艺师,E-mail:21975422@qq.com
作者简介:符伟,博士,研究员,E-mail:29313189@qq.com;
基金资助:
国家重点研发计划(2017YFD0201200);国家自然科学基金(31401775)

Resistance Development of Four Bt Insecticidal Crystal Proteins against Btk-resistant Strain of Plutella xylostella in the Laboratory

FU Wei¹, TANG Tao¹, WANG Pei¹, WANG Yong², YIN Li², MA Mingyong¹, ZHANG Zhengbing²

1. Institue of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 2. Hunan Plant Protection and Quarantine Station, Changsha 410005, China

Received:2020-12-17 Online:2022-04-08 Published:2022-04-20

摘要/Abstract

摘要： 使用苏云金芽胞杆菌库斯塔克亚种（Btk）可湿性粉剂对小菜蛾进行继代汰选获得F₈₀代和F₁₀₀代抗性品系。通过分别测定Cry1Ab、Cry1Ac、Cry1Ah和Cry1Ca四种杀虫晶体蛋白对小菜蛾Btk抗性品系F₈₀代和F₁₀₀代的室内毒力，明确了小菜蛾Btk抗性品系对四种Bt杀虫晶体蛋白抗性发展规律。研究结果表明，汰选至F₈₀代时，Cry1Ca对小菜蛾的毒力最高，LC₅₀约12.1 mg/L，其次为Cry1Ac，LC₅₀约47.7 mg/L；而汰选至F₁₀₀代时，仍以Cry1Ca对小菜蛾的毒力最高，LC₅₀约21.4 mg/L，其次为Cry1Ab，LC₅₀约72.2 mg/L。与相对敏感品系相比，小菜蛾抗性品系对Cry1Ac的抗性发展较快（抗性倍数高达67.3～106.8倍），对Cry1Ab的次之（抗性倍数高达60.0～66.1倍），而对Cry1Ca和Cry1Ah的抗性发展较慢（抗性倍数分别为3.4～6.0倍和1.6～2.5倍）。以上结果说明，在主效杀虫基因为Cry1Ac的Btk药剂选择压力下，小菜蛾对四种Bt杀虫晶体蛋白的抗性发展速度差异较大，且Cry1Ac和Cry1Ab存在交互抗性风险。

关键词: 小菜蛾, 苏云金芽胞杆菌, 杀虫晶体蛋白, 生物测定, 抗药性

Abstract: The Bacillus thuringiensis (Bt)-resistant strains of Plutella xylostella (L.) were selected using a wettable powder formulation of Bt var. kurstaki (Btk) for 80 and 100 generations in the laboratory, respectively. The toxicities of four Bt-insecticidal crystal proteins (Cry1Ab, Cry1Ac, Cry1Ah, and Cry1Ca) against Btk-resistant and -susceptible strains of P. xylostella were determined using a standard leaf-dipping method. For the F₈₀ generation of Btk-resistant strain, Cry1Ca had higher toxicity with a LC₅₀value about 12.1 mg/Lthan the other three Cry proteins, followed by Cry1Ac with a LC₅₀value about 47.7 mg/L. For the F₁₀₀ generation of Btk-resistant strain, Cry1Ca still showed higher toxicity with a LC₅₀ value about 21.4 mg/Lthan the other three Cry proteins, followed by Cry1Ab with a LC₅₀ value about 72.2 mg/L. The Btk-resistant strains of P. xylostella showed quick development of resistance to Cry1Ac, with a resistance ratio (RR) increased from 67.3-fold to 106.8-fold when compared with the susceptible laboratory strain. The resistance to Cry1Ab was relatively low, with a RR value from 60.0-fold to 66.1-fold, and the resistance to Cry1Ca and Cry1Ah was even lower, with RR values of 3.4—6.0-fold and 1.6—2.5-fold, respectively. In conclusion, these results suggest that, under the selection pressure of Btk with a dominant gene of Cry1Ac, P. xylostella strains show significant differences in the development of resistance to four insecticidal crystal proteins, and that there is a potential risk of cross-resistance between Cry1Ac and Cry1Ab.

Key words: Plutella xylostella, Bacillus thuringiensis, insecticidal crystal protein, bioassay, resistance

中图分类号:

S476.1

符伟, 唐涛, 王培, 王勇, 尹丽, 马明勇, 张政兵. 小菜蛾Btk抗性品系对四种Bt杀虫晶体蛋白抗性发展的研究[J]. 中国生物防治学报, 2022, 38(2): 328-332.

FU Wei, TANG Tao, WANG Pei, WANG Yong, YIN Li, MA Mingyong, ZHANG Zhengbing. Resistance Development of Four Bt Insecticidal Crystal Proteins against Btk-resistant Strain of Plutella xylostella in the Laboratory[J]. Chinese Journal of Biological Control, 2022, 38(2): 328-332.

参考文献

[1] Talekar N S, Shelton A M. Biology, ecology, and management of the diamondback moth[J]. Annual Review of Entomology, 1993, 38: 275-301.
[2] Furlong M J, Wright D J, Dosdall L M. Diamondback moth ecology and management: problems, progress, and prospects[J]. Annual Review of Entomology, 2013, 58: 517-541.
[3] Zalucki M P, Shabbir A, Silva R, et al. Estimating the economic cost of one of the world’s major insect pests, Plutella xylostella (Lepidoptera: Plutellidae): just how long is a piece of string?[J]. Joural of Economic Entomology, 2012, 105: 1115-1129.
[4] 李振宇, 肖勇, 吴青君, 等. 小菜蛾种群灾变及抗药性治理研究进展[J]. 应用昆虫学报, 2020, 57(3): 549-567.
[5] Schnepf E, Crickmore N, Van Rie J, et al. Bacillus thuringiensis and its pesticidal crystal proteins[J]. Microbiology and Molecular Biology Reviews, 1998, 62(3): 775-806.
[6] Sanahuja G, Banakar R, Twyma R M, et al. Bacillus thuringiensis: a century of research, development and commercial applications[J]. Plant Biotechnol Journal, 2011, 9: 283-300.
[7] Tabashnik B E, Cushing N L, Finson N, et al. Field development of resistance to Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae)[J]. Journal of Economic Entomology, 1990, 83(5): 1671-1676.
[8] 戴承镛, 殷向东, 徐熙, 等. 小菜蛾对苏云金杆菌制剂的抗药性及其对策[J]. 生物防治通报, 1994, 10(2): 62-66.
[9] Pardo-Lopez L, Sober M, Bravo A. Bacillus thuringiensis insecticidal three-domain Cry toxins: mode of action, insect resistance and consequences for crop protection[J]. FEMS Microbiology Reviews, 2013, 37(1): 3-22.
[10] 王崇利, 武淑文, 杨亦桦, 等. 东南沿海地区小菜蛾对Bt δ-内毒素和Bt制剂的抗性检测[J]. 昆虫学报, 2006, 49(1): 70-73.
[11] Wafa J, Fatma D, Luc F, et al. Review on biopesticide production by Bacillus thuringiensis subsp. kurstaki since 1990: Focus on bioprocess parameters[J]. Process Biochemistry, 2020, 98: 224-232.
[12] Cao J, Shelton A M, Earle E D. Development oftransgenic collards (Brassica oleracea L., var. acephala) expressing a cry1Ac or cry1C Bt gene for control of the diamondback moth[J]. Crop Protection, 2005, 24: 804-814.
[13] 朱勋. 抗Cry1Ac杀虫晶体蛋白近等基因系小菜蛾中肠蛋白质基因组学研究[D]. 武汉: 华中农业大学, 2014.
[14] Tabashnik B E, Cushing N L, Finson N, et al. Field development of resistance to Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae)[J]. Journal of Economic Entomology, 1990, 83(5): 1671-1676.
[15] Halpin C. Gene stacking in transgenic plants–the challenge for 21st century plant biotechology[J]. Plant Biotechnology Journal, 2005, 3: 141-155.
[16] Peterson B, Bezuidenhout C C, Van den Berg J. An overview of mechanisms of cry toxin resistance in lepidopteran insects[J]. Journal of Economic Entomology, 2017, 110(2): 362-377.
[17] Guo Z J, Kang S, Zhu X, et al. The midgut cadherin-like gene is not associated with resistance to Bacillus thuringiensis toxin Cry1Ac in Plutella xylostella (L.)[J]. Journal Invertebrate Pathology, 2015, 126: 21-30.
[18] 赵建国, 李亚新. 害虫对苏云金杆菌（Bt）制剂的抗药性及其治理策略[J]. 农药科学与管理, 1993(2): 16-18.
[19] Cao J, Shelton A M, Earle E D. Sequential transformation to pyramid two Bt genes in vegetable Indian mustard (Brassica juncea L.) and its potential for control of diamondback moth larvae[J]. Plant Cell Reports, 2008, 27: 479-487.

小菜蛾Btk抗性品系对四种Bt杀虫晶体蛋白抗性发展的研究

Resistance Development of Four Bt Insecticidal Crystal Proteins against Btk-resistant Strain of Plutella xylostella in the Laboratory

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