假格里尼翁苍白杆菌A-29对南方根结线虫的防治效果及其鉴定

doi:10.16409/j.cnki.2095-039x.2022.03.008

中国生物防治学报 ›› 2022, Vol. 38 ›› Issue (2): 367-373.DOI: 10.16409/j.cnki.2095-039x.2022.03.008

假格里尼翁苍白杆菌A-29对南方根结线虫的防治效果及其鉴定

姚亚楠¹, 丰叶青¹, 耿晶晶¹, 徐玉梅¹, 赵增旗^1,2, 王建明¹

1. 山西农业大学植物保护学院，晋中 030801；2.新西兰土地环境保护研究所，奥克兰 1072

收稿日期:2021-06-12 出版日期:2022-04-08 发布日期:2022-04-20
通讯作者: 徐玉梅,博士,教授,E-mail:ymxu@sxau.edu.cn
作者简介:姚亚楠,硕士,E-mail:yqyao0811@163.com
基金资助:
国家自然科学基金(31801958);山西省高等学校优秀成果培育项目(2019KJ021);山西省回国留学人员科研项目(HGKY2019043);山西省现代农业产业技术体系建设专项资金(2020-04);山西省中央引导地方科技发展资金(YDZJSX2021A033)

Control Efficacy of Ochrobactrum pseudogrignonese A-29 against Southern Root-knot Nematode and Its Identification

YAO Yanan¹, FENG Yeqing¹, GENG Jingjing¹, XU Yumei¹, ZHAO Zengqi^1,2, WANG Jianming¹

1. College of Plant Protection, Shanxi Agricultural University, Jinzhong 030801, China; 2. Manaaki Whenua-Landcare Research, Auckland 1072, New Zealand

Received:2021-06-12 Online:2022-04-08 Published:2022-04-20

摘要/Abstract

摘要： 南方根结线虫是一种重要的植物寄生线虫，严重发生时可造成巨大的经济损失。本研究测定了菌株A-29发酵液对南方根结线虫的致死效果和盆栽防效，并对其进行了种类鉴定。结果表明，菌株A-29发酵滤液原液、5倍液和10倍液处理南方根结线虫后，2龄幼虫的致死率在24 h和48 h后达71.09%～95.45%，卵的孵化抑制率4 d时为59.25%～87.86%，其死亡率随处理浓度的提高而增加；菌株A-29发酵液处理60 d后对番茄根结线虫的防治效果达75.00%，番茄植株鲜重、根系干重和植株高度分别增加38.41%、33.52%和20.01%；根据菌株A-29的形态特征、生理生化特征、16S rRNA基因序列及其系统发育关系，将其鉴定为假格里尼翁苍白杆菌Ochrobactrum pseudogrignonense。研究表明，菌株A-29不仅对南方根结线虫具有良好的致死效果，而且对番茄植株具有明显的促生作用，是一株具有应用前景的生防菌。

关键词: 南方根结线虫, 假格里尼翁苍白杆菌, 防治效果

Abstract: The southern root-knot nematode, Meloidogyne incognita is one of the most damaging plant-parasitic nematodes worldwide, and it can cause severe economic losses to agriculture. In this study, the antagonistic activity of strain A-29 fermentation broth against M. incognita was evaluated in vitro and in pot experiment. Furthermore, the taxonomic species of strain A-29 was identified. Four treatments were applied on second-stage juveniles (J2s) and eggs as follows: 1 x, 5 x, 10 x dilution of the fermentation filtrate of strain A-29 and a control. The fermentation filtrate of the strain A-29 showed significant potential nematicidal activities on J2s, and the remarkable inhibitory effects on egg hatching. The mortality of J2s was ranged from 71.09% to 95.45% after 24 h and 48 h; and the egg hatching inhibition was 59.25% to 87.86% after 4 d. Furthermore, the mortality percentage of J2 and eggs increased as the increase of fermentation concentration. After 60 days, the tomato seedlings with A-29 fermentation broth treatment in the pot experiment, the control efficacy was up to 75.00%, and the whole fresh weight, root dry weight and height of tomato plants were increased by 38.41%, 33.52% and 20.01%, respectively, when compared with the control. In addition, the taxonomic status of the strain A-29 was investigated and identified as Ochrobactrum pseudogrignonese based on morphological, physiological and biochemical characteristics, 16S rRNA gene sequence and Bayesian phylogenetic analysis. The results showed that O. pseudogrignonese strain A-29 exhibited high antagonistic activity against M. incognita in vitro and pot experiments, and can increase the tomato plant biomass. It is suggesting that O. pseudogrignonese strain A-29 could be considered as a potentially new biological nematicide for the control of southern root-knot nematode.

Key words: southern root-knot nematode, Ochrobactrum pseudogrignonese, control efficacy

中图分类号:

S476.2

姚亚楠, 丰叶青, 耿晶晶, 徐玉梅, 赵增旗, 王建明. 假格里尼翁苍白杆菌A-29对南方根结线虫的防治效果及其鉴定[J]. 中国生物防治学报, 2022, 38(2): 367-373.

YAO Yanan, FENG Yeqing, GENG Jingjing, XU Yumei, ZHAO Zengqi, WANG Jianming. Control Efficacy of Ochrobactrum pseudogrignonese A-29 against Southern Root-knot Nematode and Its Identification[J]. Chinese Journal of Biological Control, 2022, 38(2): 367-373.

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假格里尼翁苍白杆菌A-29对南方根结线虫的防治效果及其鉴定

Control Efficacy of Ochrobactrum pseudogrignonese A-29 against Southern Root-knot Nematode and Its Identification

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