草莓枯萎病拮抗细菌的筛选及其防治效果

doi:10.16409/j.cnki.2095-039x.2022.02.029

摘要/Abstract

摘要： 为防控草莓枯萎病，本研究通过测定生物量、初筛、复筛和相容性试验，筛选出生长速度较快且抑菌效果良好的菌株SA-1和SV-2，经形态观察、Biolog和分子生物学方法分别鉴定为枯草芽胞杆菌Bacillus subtilis和解淀粉芽胞杆菌Bacillus amyloliquefaciens。盆栽试验评价了菌株SA-1和SV-2发酵液单一使用及联合使用对草莓枯萎病的防治效果及对草莓植株促生和相关酶活的影响，结果表明菌株SA-1和SV-2发酵液联合使用比单一使用具有更好的生防效果，灌根40 d后防治效果为71.33%，过氧化氢酶活性显著增强，丙二醛含量明显减少，在草莓枯萎病生物防治中有一定应用潜力。

关键词: 草莓枯萎病, 生物防治, 芽胞杆菌

Abstract: To screen the strains against Fusarium wilt of strawberry, two strains named SA-1 and SV-2 with fast growth rate were obtained. Based on morphological observation, Biolog automatic microbial analysis and molecular identification, SA-1 and SV-2 were identified as Bacillus subtilis and Bacillus amyloliquefaciens, respectively. The effect of combination treatment with SA-1 and SV-2 on strawberry Fusarium wilt was 71.33% in pot experiment. After 40 days growth, the catalase activities of strawberry were significantly increased and the malondialdehyde were significantly decreased in the treatment with SA-1 and SV-2. These results indicated that strains SA-1 and SV-2 had potential in controlling Fusarium wilt of strawberry.

Key words: strawberry Fusarium wilt, biological control, Bacillus sp.

中图分类号:

S476

宋新娜, 梅凤珍, 李香风, 朱英波, 史凤玉, 刘建斌. 草莓枯萎病拮抗细菌的筛选及其防治效果[J]. 中国生物防治学报, 2022, 38(3): 653-661.

SONG Xinna, Mei Feng zhen, LI Xiangfeng, ZHU Yingbo, SHI Fengyu, LIU Jianbin. Screening and Control Effect of Antagonistic Bacteria against Strawberry Fusarium Wilt[J]. Chinese Journal of Biological Control, 2022, 38(3): 653-661.

参考文献

[1] Wiwiek H, Malek M, Surono S, et al. The effectiveness of a dark septate endophytic fungus, Cladophialophora chaetospira SK51, to mitigate strawberry Fusarium wilt disease and with growth promotion activities[J]. Frontiers in Microbiology, 2020, 11(1):585-595.
[2] 冶福春, 马文林, 杨晓龙. 枯草芽胞杆菌Qh-618对燕麦叶斑病防治效果研究[J]. 中国生物防治学报, 2021, 37(4):785-795.
[3] 张涛, 李雪艳, 杨红梅, 等. 4株拮抗细菌对棉花黄萎病的防治效果及机制[J]. 中国生物防治学报, 2018, 34(6):882-889.
[4] 程欢欢, 余水, 姚伟伟, 等. 辣椒炭疽病生防芽胞杆菌的筛选及田间防效[J]. 河南农业大学学报, 2019, 53(4):568-573.
[5] Liu C, Yin X H, Wang Q G, et al. Antagonistic activities of volatiles produced by two Bacillus strains against Monilinia fructicola in peach fruit[J]. Journal of the Science of Food and Agriculture, 2018, 98(15):5756-5763.
[6] Singh S, Dikshit P K, Moholkar V S, et al. Purification and characterization of acidic cellulase from Bacillus amyloliquefaciens SS35 for hydrolyzing Parthenium hysterophorus biomass[J]. Environmental Progress and Sustainable Energy, 2015, 34(3):810-818.
[7] 陈志谊, 刘永峰, 刘邮洲, 等. 植物病害生防芽胞杆菌研究进展[J]. 江苏农业学报, 2012, 28(5):999-1006.
[8] James G, Bini C D, Jose S, et al. Bacillus as an aquaculture friendly microbe[J]. Aquaculture International, 2021, 29(1):323-353.
[9] Mathurot C, Somporn C, Saisamorn L. Screening siderophore producing bacteria as potential biological control agent for fungal rice pathogens in Thailand[J]. World Journal of Microbiology and Biotechnology, 2009, 25(11):1919-1928.
[10] Dietel K, Beator B, Budiharjo A, et al. Bacterial traits involved in colonization of arabidopsis thaliana roots by Bacillus amyloliquefaciens FZB42[J]. The Plant Pathology Journal, 2013, 29(1):59-66.
[11] 马佳, 李颖, 胡栋, 等. 芽胞杆菌生物防治作用机理与应用研究进展[J]. 中国生物防治学报, 2018, 34(4):639-648.
[12] Gao Z F, Zhang B J, Liu H P, et al. Identification of endophytic Bacillus velezensis ZSY-1 strain and antifungal activity of its volatile compounds against Alternaria solani and Botrytis cinerea[J]. Biological Control, 2017, 105(1):27-39.
[13] Chen X H, Koumoutsi A, Scholz R, et al. Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens[J]. Journal of Biotechnology, 2008, 140(1-2):27-37.
[14] Chen X H, Koumoutsi A, Scholz R, et al. More than anticipated-production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42[J]. Journal of Molecular Microbiology and Biotechnology, 2009, 16(1-2):14-24.
[15] 要雅倩, 成娜娜, 李培根, 等. 解淀粉芽胞杆菌T-6的分离鉴定及抗病促生潜力[J]. 生物技术通报, 2020, 36(9):202-210.
[16] 余水, 丁海霞, 罗玉英, 等. 解淀粉芽胞杆菌MT323生防机制初探[J]. 贵州农业科学, 2020, 48(1):59-62.
[17] Ahmed K E, Sajeewa M, Mohammed A A, et al. Biocontrol potential of Bacillus amyloliquefaciens against Botrytis pelargonii and Alternaria alternata on Capsicum annuum[J]. Journal of Fungi, 2021, 7(6):472-472.
[18] 赵卫松, 郭庆港, 张晓云, 等. 解淀粉芽胞杆菌PHODG36菌剂的研制及其对马铃薯黄萎病的防病增产效果[J].中国生物防治学报, 2020, 36(03):381-387.
[19] Chowdhury S P, Dietel K, Randler M, et al. Effects of Bacillus amyloliquefaciens FZB42 on lettuce growth and health under pathogen pressure and its impact on the rhizosphere bacterial community[J]. PLoS ONE, 2013, 8(7):1-10.
[20] 赵雨萌, 缪佩佩, 王旭东, 等. 解淀粉芽胞杆菌TR2对草莓土壤酶活性的影响与防病促生作用[J]. 中国生物防治学报, 2022, 38(2):495-501.
[21] 乔欣蕾, 凡雪蕊, 霍晓毅, 等. 枯草芽胞杆菌拮抗尖孢镰刀菌的抑菌物质分析[J]. 农业生物技术学报, 2021, 29(10):1999-2007.
[22] 赵卫松, 鹿秀云, 郭庆港, 等. 防治番茄灰霉病的枯草芽胞杆菌BAB-1粉尘剂研制[J]. 中国生物防治学报, 2018, 34(1):99-108.
[23] 揣红运, 谢学文, 石延霞, 等. 枯草芽胞杆菌微粉剂的研制及其对黄瓜白粉病的防治效果[J]. 植物病理学报, 2019, 49(5):660-669.
[24] 陈川雁, 王燕, 喻国辉, 等. 枯草芽胞杆菌R31影响巴西蕉根系活性氧产生及对枯萎病的防治效果[J]. 中国生物防治学报, 2017, 33(02):226-233.
[25] 赵新贝, 王娟, 上官妮妮, 等. 番茄灰霉病生防细菌TD-7的鉴定, 发酵条件优化及其防治效果[J]. 中国生物防治学报, 2019, 35(2):226-239.
[26] Choi T G, Maung C H, Lee D R, et al. Role of bacterial antagonists of fungal pathogens, Bacillus thuringiensis KYC and Bacillus velezensis CE100 in control of root-knot nematode, Meloidogyne incognita and subsequent growth promotion of tomato[J]. Biocontrol Science and Technology, 2020, 30(7):685-700.
[27] 付莉媛. 北京地区草莓根腐病致病菌的分离鉴定及拮抗菌筛选[D]. 秦皇岛:河北科技师范学院, 2021.
[28] 冯志敏. 生防芽胞杆菌的筛选及其对葡萄灰霉病的防控研究[D]. 秦皇岛:河北科技师范学院, 2019.
[29] Barbosa L O, Lima J S, Magalhaes V C, et al. Compatibility and combination of selected bacterial antagonists in the biocontrol of sisal bole rot disease[J]. Biological Control, 2018, 63:595-605.
[30] 胡桂林, 王德良, 张雪峰, 等. 用Biolog微生物自动分析系统鉴定大曲中地衣芽孢杆菌的研究[J].酿酒, 2007(6):32-33.
[31] 顾春波, 史晓斌, 姜莉莉, 等. 草莓枯萎病菌对多菌灵的抗性及其抗性菌株生物学特性[J]. 植物保护学报, 2010, 37(3):266-272.
[32] 陈秀娟, 陈卫平, 糜林, 等. 南方草莓叶面积计算方法的研究[J]. 中国农学通报, 2009, 25(14):190-193.
[33] 石妞妞, 杜宜新, 阮宏椿, 等. 枯草芽胞杆菌T122F内生定殖及对香蕉枯萎病的防治效果[J]. 植物保护, 2015, 41(4):95-99,124.
[34] 高毓晗, 李世东, 郭荣君. sfp基因转化增强了Bacillus subtilis 168的定殖能力和对黄瓜茎内枯萎病菌的抑制作用[J]. 中国生物防治学报, 2016, 32(01):76-85.
[35] 王诗晗, 鲁琴, 鲍佳楠, 等. 向日葵枯萎病拮抗芽胞杆菌的筛选与生防评价[J]. 中国微生态学杂志, 2020, 32(01):6-10,16.
[36] 王晓丽. 马铃薯枯萎病发生特点及防治措施的初步研究[D]. 呼和浩特:内蒙古农业大学, 2012.
[37] 王璐瑶, 李兴东, 段天凤, 等. 解淀粉芽胞杆菌B1619防控设施番茄枯萎病田间使用技术研究与示范[J]. 中国生物防治学报, 2017, 33(4):512-518.
[38] 王亚娇, 栗秋生, 纪莉景, 等. 一株西瓜枯萎病生防菌的鉴定与田间防效[J]. 微生物学通报, 2021, 48(6):1976-1984.
[39] 陈燕红, 陈远凤, 黎永坚, 等. 一株香蕉枯萎病生防解淀粉芽胞杆菌的分离、鉴定及其拮抗物质研究[J].广东农业科学, 2013, 40(2):68-72.
[40] 崔宁. 拮抗细菌的分离鉴定及其对苦瓜枯萎病生防潜力的研究[D]. 泰安:山东农业大学, 2017.
[41] 丁建朋, 范瑛阁, 姚永生. 生防菌HFW217的鉴定及其对棉花枯萎病的防治效果[J]. 新疆农业科学, 2019, 56(03):498-508.