两种果蝇对不同成熟期草莓果实及其挥发物的趋性行为分析

doi:10.16409/j.cnki.2095-039x.2022.11.016

摘要/Abstract

摘要： 黑腹果蝇Drosophila melanogaster和斑翅果蝇D. suzukii能够为害包括草莓Fragaria×ananassa在内的多种浆果类经济水果,造成巨大经济损失。本研究通过分析这两种果蝇对不同成熟期草莓及其挥发物组分的趋性行为,旨在为果蝇引诱剂的研发筛选有应用价值的挥发物组分。双选择陷阱试验结果表明,相较于琼脂凝胶,各个成熟期的草莓对两种果蝇雌成虫均有显著的吸引效果。两种果蝇对不同成熟期草莓的选择偏好依次为腐坏草莓,红熟草莓和白熟草莓;红熟期和白熟期的草莓对果蝇的吸引效果无显著差异。通过HS-SPME和GC-MS收集分析了不同成熟期草莓的挥发物,发现红熟期和腐坏期草莓挥发物组分差异显著。在5种测试的化合物中,仅乙酸乙酯对两种果蝇雌虫有显著吸引效果,效应浓度为0.005%~0.05%(v/v)。

关键词: 黑腹果蝇, 斑翅果蝇, 草莓, 挥发物, 趋性行为

Abstract: Drosophila melanogaster and D. suzukii attack a variety of economic soft fruits including strawberries Fragaria×ananassa, resulting in huge economic loss. The orientation behaviors of the two Drosophila species to fruits and volatiles of strawberries at different mature stages were analyzed in the present study to screen valuable compounds for the development of fruit fly attractant. Two-choice trap-based bioassays showed that strawberries at all the three mature stages were significantly more attractive to female adults of the two Drosophila species than agar gel. The preference of D. melanogaster and D. suzukii to strawberries at different mature stages ranked from high to low was overripe fruits, red-ripe fruits and white-ripe fruits, but no significant difference was found in the attractiveness between red-ripe fruits and white-ripe fruits. The volatiles from strawberries at different mature stages were collected and analyzed by HS-SPME and GC-MS. It was found that the volatile components from strawberries at red-ripe stage and overripe stage were significantly different. Among the 5 compounds tested, only ethyl acetate had significant attractiveness to the two species of Drosophila females, with effective concentrations ranging from 0.005%-0.05% (v/v).

Key words: Drosophila melanogaster, Drosophila suzukii, strawberry, volatile, tropism behavior

中图分类号:

S436.63

张璇, 毕亚銮, 方琦, 汪芳, 叶恭银. 两种果蝇对不同成熟期草莓果实及其挥发物的趋性行为分析[J]. 中国生物防治学报, 2023, 39(4): 813-823.

ZHANG Xuan, BI Yaluan, FANG Qi, WANG Fang, YE Gongyin. Tropism Behavior Analysis of Two Drosophila Species to Fruits and Volatiles of Strawberry at Different Mature Stages[J]. Chinese Journal of Biological Control, 2023, 39(4): 813-823.

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