短时低温胁迫对日本食蚧蚜小蜂存活、发育及繁殖的影响

doi:10.16409/j.cnki.2095-039x.2022.11.025

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (5): 1029-1037.DOI: 10.16409/j.cnki.2095-039x.2022.11.025

短时低温胁迫对日本食蚧蚜小蜂存活、发育及繁殖的影响

杨美娟^1,2, 陈俊谕², 叶政培², 符悦冠², 王建赟², 朱俊洪¹, 张方平²

1. 海南大学植物保护学院/热带农林生物灾害绿色防控教育部重点实验室, 海口 570228;
2. 中国热带农业科学院环境与植物保护研究所/海南省热带作物病虫害生物防治工程技术研究中心/农业农村部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室, 海口 571101

收稿日期:2022-08-01 出版日期:2023-10-08 发布日期:2023-10-25
通讯作者: 朱俊洪,副教授,E-mail:junhonz@163.com;张方平,研究员,E-mail:fangpingz97@163.com。
作者简介:杨美娟,硕士研究生,E-mail:meijuany@163.com。
基金资助:
海南省自然科学基金（321RC458，321RC623）；国家现代农业产业技术体系（CARS-33-GW-BC2）

Effects of Short-term Low Temperature Stress on Survival, Development and Reproduction of the Coccophagus japonicus Compere

YANG Meijuan^1,2, CHEN Junyu², YE Zhengpei², FU Yueguan², WANG Jianyun², ZHU Junhong¹, ZHANG Fangping²

1. College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, Haikou 570228, China;
2. Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests/Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests/Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China

Received:2022-08-01 Online:2023-10-08 Published:2023-10-25

摘要/Abstract

摘要： 为探明短时低温胁迫对日本食蚧蚜小蜂Coccophagus japonicus Compere存活、发育及繁殖的影响，本研究观察了日本食蚧蚜小蜂幼虫、蛹在2、4、6 ℃条件下分别胁迫0.5、1、2 d后的存活率、发育历期、初羽化雌蜂的体长、成熟卵量及其雌成蜂寿命，并研究了成蜂在2、4、6 ℃条件下的存活情况。结果表明：日本食蚧蚜小蜂幼虫和蛹的存活率、初羽化雌蜂的体长及其成熟卵量、雌成蜂寿命均与其经历的低温胁迫温度呈正相关，与胁迫时间呈负相关；发育历期与低温胁迫温度呈负相关，与胁迫时间呈正相关，表现为随着温度降低和胁迫时间延长，日本食蚧蚜小蜂幼虫、蛹的存活率下降，发育历期延长，初羽化雌蜂的体长缩短，成熟卵量减少，雌成蜂寿命降低；在6、4、2 ℃条件下胁迫日本食蚧蚜小蜂成蜂其致死中时间随温度的下降而缩短，分别是4.88、3.39、1.64 d，均明显低于27 ℃。综上，短时低温胁迫对日本食蚧蚜小蜂的种群增长不利，田间应用该蜂防治橡副珠蜡蚧Parasaissetia nigra Nietner时需考虑释放地的年度低温及持续时间进行适当补充放蜂。

关键词: 日本食蚧蚜小蜂, 短时低温胁迫, 存活, 发育, 繁殖

Abstract: This study aims to investigate the effects of short-term low-temperature stress on the survival, development and reproduction of Coccophagus japonicus Compere. Larvae and pupae of the wasps were stressed at three temperature levels, 2, 4 and 6 ℃ for 0.5, 1 and 2 d, respectively. The survival rate, developmental duration, body length, initial egg load, and female longevity of these stressed C. japonicus were assessed. Additionally, the resulting adults were exposed to 2, 4 and 6 ℃ for survival analysis. The results showed that the survival rates of larvae and pupae, the body length, the initial egg load, and the female longevity of C. japonicus were positively correlated with the temperature and negatively correlated with the duration of stress, when the wasps experienced at their larva- or pupa-stage. Also, the developmental duration was negatively correlated with the temperature and positively correlated with the duration of stress. With the decrease of temperature and the prolongation of stress time, the survival rate of larvae and pupae of C. japonicus decreased, the developmental period was prolonged, the body length was shortened, the initial egg load and the female longevity were reduced. When the adults experienced the low temperatures (2, 4 and 6 ℃), the LT₅₀was significantly shorter than that at 27 ℃. The LT₅₀of adults decreased with the decrease of temperature, being 4.88, 3.39 and 1.64 d at 6, 4 and 2 ℃, respectively. In conclusion, the short-term low temperature stress is detrimental to the population growth of the C. japonicus. Therefore, the annual low temperature and duration should be considered when applying this wasp in the field for control of Parasaissetia nigra Nietner.

Key words: Coccophagus japonicus, short-term low temperature stress, survival, development, reproduction

中图分类号:

S476.3

杨美娟, 陈俊谕, 叶政培, 符悦冠, 王建赟, 朱俊洪, 张方平. 短时低温胁迫对日本食蚧蚜小蜂存活、发育及繁殖的影响[J]. 中国生物防治学报, 2023, 39(5): 1029-1037.

YANG Meijuan, CHEN Junyu, YE Zhengpei, FU Yueguan, WANG Jianyun, ZHU Junhong, ZHANG Fangping. Effects of Short-term Low Temperature Stress on Survival, Development and Reproduction of the Coccophagus japonicus Compere[J]. Chinese Journal of Biological Control, 2023, 39(5): 1029-1037.

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短时低温胁迫对日本食蚧蚜小蜂存活、发育及繁殖的影响

Effects of Short-term Low Temperature Stress on Survival, Development and Reproduction of the Coccophagus japonicus Compere

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