番茄潜叶蛾原产地潜叶蛾伲姬小蜂对入侵地4种非靶标昆虫的寄主专一性风险评价

doi:10.16409/j.cnki.2095-039x.2025.09.025

中国生物防治学报 ›› 2026, Vol. 42 ›› Issue (5): 1055-1063.DOI: 10.16409/j.cnki.2095-039x.2025.09.025

• 研究论文 •

番茄潜叶蛾原产地潜叶蛾伲姬小蜂对入侵地4种非靶标昆虫的寄主专一性风险评价

张桂芬¹, 王浩¹, 张毅波¹, 冼晓青¹, 黄聪¹, 李涛², COLLATZ Jana³, ARNÓ Judit⁴, 万方浩¹

1. 中国农业科学院植物保护研究所/植物病虫害综合治理全国重点实验室/农业农村部外来入侵生物防控重点实验室, 北京 100193;
2. 新疆生产建设兵团第十四师农业科学研究所, 昆玉 848116;
3. 瑞士农业生态与环境研究部农业科学研究院, 苏黎世 8046;
4. 西班牙农业食品研究与技术研究所, 卡布里尔斯 08348

收稿日期:2025-11-03 发布日期:2026-10-21
通讯作者: 张桂芬,博士,研究员,E-mail:zhangguifen@caas.cn。
作者简介:张桂芬,博士,研究员,E-mail:zhangguifen@caas.cn;王浩,硕士研究生,E-mail:wh691827@163.com;张毅波,博士,研究员,E-mail:zhangyibo@caas.cn。
基金资助:
国家重点研发计划（2021YFD1400200）；中国农业科学院科技创新工程（caascx-2021-2025-IAS）；2023年兵团人才发展基金“天山英才”支持计划（“三农”骨干人才项目）

Host Specificity Risk Assessment of Necremnus tutae Ribes & Bernardo (Hymenoptera: Eulophidae), a Parasitoid Wasp from the Native Range of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), on Four Non-Target Insect Species in Invaded Agroecosystems

ZHANG Guifen¹, WANG Hao¹, ZHANG Yibo¹, XIAN Xiaoqing¹, HUANG Cong¹, LI Tao², COLLATZ Jana³, ARNÓ Judit⁴, WAN Fanghao¹

1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory for Prevention and Control of Invasive Alien Species of Ministry of Agriculture and Rural Affairs/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. The Agricultural Science Research Institute of the 14th Division, Xinjiang Production and Construction Corps, Kunyu 848116, China;
3. Agroscope, Research Division Agroecology and Environment, Zurich 8046, Switzerland;
4. Institute for Research and Technology in Agriculture (IRTA), Cabrils 08348, Spain

Received:2025-11-03 Published:2026-10-21

摘要/Abstract

摘要： 番茄潜叶蛾已成为我国南北方番茄田的主要害虫，目前已在20多个省级区域发生。潜叶蛾伲姬小蜂为寄主取食型寄生蜂，广泛分布于南美洲、北美洲和欧洲，是番茄潜叶蛾的专性寄生蜂，于2019年引入我国。为了防止新的入侵事件发生，本研究以番茄田常见的4种非靶标种昆虫/害虫为测试对象，在室内非选择条件下开展潜叶蛾伲姬小蜂的寄主专一性风险评价研究。结果表明，潜叶蛾伲姬小蜂可以取食和叮蛰美洲斑潜蝇2龄幼虫和斜纹夜蛾1龄幼虫，48 h总致死数分别为2.50头和1.20头，总致死率分别为12.50%和6.00%；但是，潜叶蛾伲姬小蜂对二者均不具有产卵寄生行为。并且，该种寄生蜂对新近入侵我国的草地贪夜蛾1龄和2龄幼虫，以及瓜蚜成虫不具有任何致死行为（包括寄生、取食、叮蛰），全部表现为拒绝。进一步对产卵寄生风险指数（RI）和寄主专一性综合风险指数（CRI）进行计算分析，显示，潜叶蛾伲姬小蜂对4种非靶标种昆虫的产卵寄生风险指数RI值均为0，寄主专一性综合风险指数CRI值亦均为0；表明，潜叶蛾伲姬小蜂对上述4种非靶标种昆虫均属0风险，可作为番茄潜叶蛾的安全高效生防作用物。研究结果对番茄潜叶蛾综合治理中可持续利用和规范释放潜叶蛾伲姬小蜂提供了科学指导。

关键词: 番茄潜叶蛾, 潜叶蛾伲姬小蜂, 非靶标风险, 寄主专一性, 风险评估

Abstract: The tomato leafminer, Tuta absoluta (Meyrick), has become a major pest in tomato fields across both northern and southern China, with reported occurrences in over 20 provincial-level regions. Necremnus tutae Ribes & Bernardo, a host-feeding parasitoid native to South America and Europe, is a specialized natural enemy of T. absoluta and was introduced into China in 2019. To prevent potential non-target effects and ensure ecological safety following its release, we evaluated the host specificity of N. tutaeunder laboratory no-choice conditions, targeting four common non-target insect/pest species in tomato agroecosystems, which included two invasive species, the leafminer Liriomyza sativae (Blanchard) and the fall armyworm Spodoptera frugiperda (Smith), and two native species, the common cutworm Spodoptera litura (Fabricius) and the melon aphid Aphis gossypii (Glover). Behavioral assays revealed that N. tutaeexhibited limited predatory and stinging behaviors toward the 2^nd-instar larvae of L. sativae and the 1^st-instar larvae of S. litura, with total number of killed pests of 2.5 and 1.2 within 48 hours, corresponding to mortality rates of 12.5% and 6.0%, respectively. However, no oviposition behavior was observed on either species. Notably, N. tutaedisplayed complete rejection of the 1^st- and 2^nd-instar larvae of S. frugiperda and the adults of A. gossypii, with no killing behaviors (including parasitism, feeding, or stinging) detected. To quantify the ecological risk, two indices were calculated: the Reproductive Parasitism Risk Index (RI) and the Comprehensive Host Specificity Risk Index (CRI). Results showed that both RI and CRI values were 0 for all four non-target species, confirming that N. tutaeposes negligible ecological risk or risk-free to the tested four non-target organisms, supporting its safety for field release. This study provides critical insights into the host specificity of N. tutae, highlighting its potential as a safe and effective biocontrol agent against T. absoluta in China. The results offer scientific guidance for the sustainable utilization and standardized release of N. tutaein integrated pest management (IPM) of T. absoluta.

Key words: Tuta absoluta, Necremnus tutae, non-target risk, host specificity, risk assessment

中图分类号:

S476.3

张桂芬, 王浩, 张毅波, 冼晓青, 黄聪, 李涛, COLLATZ Jana, ARNÓ Judit, 万方浩. 番茄潜叶蛾原产地潜叶蛾伲姬小蜂对入侵地4种非靶标昆虫的寄主专一性风险评价[J]. 中国生物防治学报, 2026, 42(5): 1055-1063.

ZHANG Guifen, WANG Hao, ZHANG Yibo, XIAN Xiaoqing, HUANG Cong, LI Tao, COLLATZ Jana, ARNÓ Judit, WAN Fanghao. Host Specificity Risk Assessment of Necremnus tutae Ribes & Bernardo (Hymenoptera: Eulophidae), a Parasitoid Wasp from the Native Range of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), on Four Non-Target Insect Species in Invaded Agroecosystems[J]. Chinese Journal of Biological Control, 2026, 42(5): 1055-1063.

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番茄潜叶蛾原产地潜叶蛾伲姬小蜂对入侵地4种非靶标昆虫的寄主专一性风险评价

Host Specificity Risk Assessment of Necremnus tutae Ribes & Bernardo (Hymenoptera: Eulophidae), a Parasitoid Wasp from the Native Range of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), on Four Non-Target Insect Species in Invaded Agroecosystems

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