皂苷胁迫后三七根系分泌物促进蒙氏假单胞菌PM41在土壤中存活的效应

doi:10.16409/j.cnki.2095-039x.2026.02.030

摘要/Abstract

摘要： 本研究旨在探究皂苷胁迫下三七根系分泌物及其所含化合物对有益菌蒙氏假单胞菌Pseudomonas monteilii PM41的诱导作用、生长、生物膜形成及土壤中种群数量的影响，揭示三七通过根系分泌物募集PM41的效应，为缓解三七连作障碍提供新策略。结果表明，皂苷胁迫下的三七根系分泌物可显著诱导PM41。进一步分析发现，其中的4-甲氧基肉桂酸和愈伤酸对该菌的诱导、生物膜形成具有显著促进效应。土壤存活验证结果显示，这两种化合物均可显著提高PM41在灭菌土壤中的存活率，分别提高77.46%和101.41%，效果优于总根系分泌物处理。综上，皂苷胁迫后的三七根系分泌物可显著促进PM41的存活与定殖，其中4-甲氧基肉桂酸和愈伤酸可能是介导该过程的关键化合物。

关键词: 皂苷, 三七, 连作障碍, 生防菌, 根系分泌物, 非生物胁迫

Abstract: This study aimed to investigate the effects of root exudates of Panax notoginseng under saponin stress and their compounds on the induction, growth, biofilm formation, and soil population dynamics of the beneficial bacterium Pseudomonas monteilii PM41, with the goal of elucidating the recruitment of PM41 by P. notoginseng root exudates and providing a novel strategy for mitigating continuous cropping obstacles in P. notoginseng. The results showed that root exudates of P. notoginseng under saponin stress significantly induced PM41. Further analysis indicated that 4-methoxycinnamic acid and traumatic acid, among the identified compounds, exerted pronounced promoting effects on the induction, and biofilm formation of this strain. Soil survival assays demonstrated that both compounds significantly increased the survival rate of PM41 in sterilized soil, with increases of 77.46% and 101.41%, respectively, outperforming the total root exudate treatment. Collectively, root exudates of P. notoginseng under saponin stress significantly promoted the survival and colonization of PM41, with 4-methoxycinnamic acid and traumatic acid potentially serving as key compounds mediating this process.

Key words: saponin, Panax notoginseng, replant failure, biocontrol bacteria, root exudates, abiotic stress

中图分类号:

S476

钟诗俊, 邓琳梅, 罗悦, 陈乐霖, 孔惟冉, 杨绍磊, 朱书生, 杨敏, 罗丽芬. 皂苷胁迫后三七根系分泌物促进蒙氏假单胞菌PM41在土壤中存活的效应[J]. 中国生物防治学报, 2026, 42(3): 648-659.

ZHONG Shijun, DENG Linmei, LUO Yue, CHEN Lelin, KONG Weiran, YANG Shaolei, ZHU Shusheng, YANG Min, LUO Lifen. Effect of Root Exudates of Panax notoginseng under Saponin Stress on Survival of Pseudomonas monteilii PM41 in Soil[J]. Chinese Journal of Biological Control, 2026, 42(3): 648-659.

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