荧光假单胞菌2P24防控瓜类果斑病机制初探

doi:10.16409/j.cnki.2095-039x.2023.02.022

中国生物防治学报 ›› 2023, Vol. 39 ›› Issue (3): 575-584.DOI: 10.16409/j.cnki.2095-039x.2023.02.022

荧光假单胞菌2P24防控瓜类果斑病机制初探

汪心玉¹, 芦钰², 邱艳红², 张海军², 张力群¹, 李健强¹, 王红阳², 罗来鑫¹, 徐秀兰²

1. 中国农业大学植物保护学院/种子病害检验与防控北京市重点实验室, 北京 100193;
2. 北京市农林科学院蔬菜研究所/农业农村部蔬菜种子质量监督检验测试中心/蔬菜生物育种全国重点实验室, 北京 100097

收稿日期:2022-07-12 出版日期:2023-06-08 发布日期:2023-06-25
通讯作者: 罗来鑫,副教授,E-mail:08030@cau.edu.cn;徐秀兰,副研究员,E-mail:xuxiuLan@nercv.org。
作者简介:汪心玉，硕士研究生，E-mail：wxyu980903@126.com。
基金资助:
北京市农林科学院创新专项（KJCX20230214，KJCX20230430）；北京市农林科学院公益检测专项（YZX002）

Preliminary Study on The Control Mechanism of Pseudomonas fluorescens 2P24 on Bacterial Fruit Blotch

WANG Xinyu¹, LU Yu², QIU Yanhong², ZHANG Haijun², ZHANG Liqun¹, LI Jianqiang¹, WANG Hongyang², LUO Laixin¹, XU Xiulan²

1. Beijing Key Laboratory of Seed Disease Testing and Control/College of Plant Pathology, China Agricultural University, Beijing 100093, China;
2. Bijing Vegetables Research Center, Beijing Academy of Agriculture and Forestry/Supervision, Inspection and Test Center of Vegetable Seed Quality of Ministry Agricultural and Rural Affair/State Key Laboratory of Vegetable Biobreeding, Beijing 100097, China

Received:2022-07-12 Online:2023-06-08 Published:2023-06-25

摘要/Abstract

摘要： 为初步解析荧光假单胞2P24对瓜类细果斑病的防控机制，测试其次生代谢物2,4-DAPG、MPC对西瓜嗜酸菌Acidovorax citrulli生长的影响，并比较分析2P24及其突变株△phlD（丧失2,4-DAPG合成能力）及△gacS（丧失2,4-DAPG、HCN、蛋白酶合成能力）的病害防控效果和对甜瓜生理生化指标的影响。结果表明，2,4-DAPG及MPC对病原菌的生长、游动性及生物膜的形成均具有抑制作用；与2P24相比，△phlD对病原菌的生长抑制降低，△gacS丧失生长抑制作用。△phlD及△gacS病害相对防治率分别下降了44.3%和77.1%。2P24对于西瓜、甜瓜幼苗有明显的促生作用，可显著提高幼苗株高和鲜重，同时发现2P24可在病原菌接种后诱导甜瓜抗性相关酶SOD、CAT、POD活性提升及3个相关抗性基因MELO3C023441、MELO3C022146及MELO3C004303的表达。本研究结果为瓜类果斑病的生物防治研究提供了参考和理论依据。

关键词: 荧光假单胞, 瓜类果斑病, 生防菌, 种传病害

Abstract: In order to explore the mechanism of Pseudomonas fluorescens 2P24 against bacterial fruit blotch, the effects of the secondary metabolites, 2,4-DAPG and MPC on the growth of the pathogen, Acidovorax citrulli were tested. The disease control efficacy of 2P24 and its mutants ΔphlD (lack of 2,4-DAPG synthesis) and ΔgacS (lack of 2,4-DAPG, HCN and protease synthesis) were compared and analyzed. The results showed that 2,4-DAPG and MPC inhibited the growth, motility, and biofilm formation of A. citrulli. Compared with 2P24, the growth inhibition effect of △phlD decreased and ΔgacS lost. The relative control efficacy of ΔphlD and ΔgacS reduced by 44.3% and 77.1%, respectively, comparing to 2P24. 2P24 promoted the growth of watermelon and melon seedlings, increased the height and fresh weight of seedlings. In addition, 2P24 improved the activity of SOD, CAT and POD of melon and increased the expression of three resistance related genes, MELO3C023441, MELO3C022146, MELO3C004303. This study provided a reference and theoretical basis for the biological control of bacterial fruit blotch.

Key words: Acidovorax citrulli, bacterial fruit blotch, biocontrol bacteria, seed-borne disease

中图分类号:

S476

汪心玉, 芦钰, 邱艳红, 张海军, 张力群, 李健强, 王红阳, 罗来鑫, 徐秀兰. 荧光假单胞菌2P24防控瓜类果斑病机制初探[J]. 中国生物防治学报, 2023, 39(3): 575-584.

WANG Xinyu, LU Yu, QIU Yanhong, ZHANG Haijun, ZHANG Liqun, LI Jianqiang, WANG Hongyang, LUO Laixin, XU Xiulan. Preliminary Study on The Control Mechanism of Pseudomonas fluorescens 2P24 on Bacterial Fruit Blotch[J]. Chinese Journal of Biological Control, 2023, 39(3): 575-584.

参考文献

[1] Webb R E, Goth R W. A seedborne bacterium isolated from watermelon[J]. Plant Disease Report, 1965, 49(8):18-21.
[2] 张兴平, Rhod B B. 一种毁灭性的西瓜新病害细菌性果实腐斑病[J]. 中国西瓜甜瓜, 1990(2):12, 44-45.
[3] 赵廷昌, 孙福在, 王兵万. 西瓜细菌性果斑病研究进展[J]. 植保技术与推广, 2001, 21(3):37-38.
[4] Márcia T C, Silveira E B D, Mariano R D L R, et al. Growth of Acidovorax avenae subsp. citrulli under different variable temperature, pH, sodium chloride concentrations and carbon sources[J]. Ciência Rural, 2005, 35(6):1313-1318.
[5] Wang T, Yang Y, Zhao T. Genome sequence of a copper-resistant strain of Acidovorax citrulli causing bacterial fruit blotch of melons[J]. Genome Announcements. 2015, 23, 3(2):e00310-15.
[6] Nadeem S M, Ahmad M, Zahir Z A, et al. The role of mycorrhizae and plant growth promoting rhizobacteria (PGPR) in improving crop productivity under stressful environments[J]. Biotechnology Advances, 2014, 32:429-448.
[7] Malusá E, Sas-Paszt L, Ciesielska J. Technologies for beneficial microorganisms inocula used as biofertilizers[J]. The Scientific World Journal, 2012(1):491206.
[8] Prabhukarthikeyan S R, Keerthana U, Raguchander T. Antibiotic-producing Pseudomonas fluorescens mediates rhizome rot disease resistance and promotes plant growth in turmeric plants[J]. Microbiological Research, 2018, 210:65-73.
[9] Peeran M F, Krishnan N, Thangamani P R, et al. Development and evaluation of water-in-oil formulation of Pseudomonas fluorescens (FP7) against Colletotrichum musae incitant of anthracnose disease in banana[J]. European Journal of Plant Pathology, 2014, 138(1):167-180.
[10] 张清霞, 吴小刚, 张力群, 等. 荧光假单胞菌2P24调控基因突变体定殖能力和生防效果分析[J]. 中国生物防治, 2008, 24(1):7.
[11] Brooks S. Napthoquinones from Neocosmospora sp.-antibiotic activity against Acidovorax citrulli, the causative agent of bacterial fruit blotch in watermelon and melon[J]. Journal of Fungi, 2021, 7(5):370.
[12] 王全亮, 于勇谋, 曹亚萍, 等. 两系混种防控小麦黄矮病技术研究[J]. 中国植保导刊, 2018, 38(1):4.
[13] 丁富功, 侯泽豪, 张迎新, 等. 玉米盛花期不同时间授粉对果穗结实性状的影响[J]. 长江大学学报:自然科学版, 2019, 16(10):6.
[14] Giannopolitis C N, Ries S K. Superoxide dismutases:I. Occurrence in higher plants[J]. Plant Physiology,1977, 59:309-314.
[15] Kato-Noguchi, H, Macías, F A. Effects of 6-methoxy-2-benzoxazolinone on the germination and α-amylase activity in lettuce seeds[J]. Journal of Plant Physiology, 2005, 162:1304-1307.
[16] Scebba F, Sebastiani L, Vitagliano C. Activities of antioxidant enzymes during senescence of Prunus armeniaca leaves[J]. Biologia Plantarum, 2001, 44:41-46.
[17] Islam M R, Hossain M R, Jesse D, et al. Characterization, identification and expression profiling of genome-wide R-genes in melon and their putative roles in bacterial fruit blotch resistance[J]. BMC Genetics, 2020, 21:80.
[18] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2- ΔΔCT method[J]. Methods, 2001, 25(4):2-8.
[19] Chitra P, Jijeesh C M. Biopriming of seeds with plant growth promoting bacteria Pseudomonas fluorescens for better germination and seedling vigour of the East Indian sandalwood[J]. New Forests, 2021, 52:829-841.
[20] Moeinzadeh A, Sharif-Zadeh F, Ahmadzadeh M, et al. Biopriming of sunflower (Helianthus annuus L.) seed with Pseudomonas fluorescens for improvement of seed invigoration and seedling growth[J]. Australian Journal of Crop Science, 2010, 4(7):564-570.
[21] 刘九成, 张伟, 吴小刚, 等. 荧光假单胞菌2P24中ret对抗生素2,4-二乙酰基间苯三酚合成的影响[J]. 微生物学报, 2013(2):118-126.
[22] Gong L, Tan H, Chen F, et al. Novel synthesized 2, 4-DAPG analogues:antifungal activity, mechanism and toxicology[J]. Scientific Reports, 2016, 6:32266.
[23] Ellis J, Dodds P, Pryor T. Structure, function and evolution of plant disease resistance genes[J]. Current Opinion in Plant Biology, 2000, 3:278-284.
[24] Raja S N, Jeffery L, Caplan K C, et al. The role of TIR-NBS and TIR-X proteins in plant basal defense responses[J]. Plant Physiology, 2013, 162:1459-1472.

荧光假单胞菌2P24防控瓜类果斑病机制初探

Preliminary Study on The Control Mechanism of Pseudomonas fluorescens 2P24 on Bacterial Fruit Blotch

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