莱氏绿僵菌Mr006和氯虫苯甲酰胺协同应用对草地贪夜蛾的毒力

doi:10.16409/j.cnki.2095-039x.2026.09.001

摘要/Abstract

摘要： 本研究评价了莱氏绿僵菌Mr006与氯虫苯甲酰胺协同使用对草地贪夜蛾的防治效果。通过毒力测定发现，莱氏绿僵菌Mr006处理7 d后对草地贪夜蛾的LC₂₅、LC₅₀和LC₉₀分别为8.97×10⁵ CFU/mL、1.12×10⁷ CFU/mL和1.37×10⁹ CFU/mL；而氯虫苯甲酰胺处理4 d后的相应LC分别为0.05 mg/L、0.24 mg/L和4.35 mg/L。相容性试验结果表明，在LC₂₅、LC₅₀和LC₉₀浓度下，氯虫苯甲酰胺对莱氏绿僵菌Mr006的孢子萌发、菌丝生长和产孢量均无显著影响。在施用顺序和间隔时间方面，所有处理均显著提高了草地贪夜蛾的校正死亡率，其中“先施用莱氏绿僵菌后施用氯虫苯甲酰胺”的处理方式表现出更强的协同增效作用，在莱氏绿僵菌处理后36 h施用农药的处理组，7 d后的校正死亡率高达92.93%。莱氏绿僵菌Mr006与氯虫苯甲酰胺以1:4比例混配时增效作用最为显著，7 d校正死亡率达到81.82%。本研究结果为草地贪夜蛾的绿色防控提供了重要的理论依据和实践指导。

关键词: 莱氏绿僵菌, 氯虫苯甲酰胺, 草地贪夜蛾, 毒力, 协同增效

Abstract: This study evaluated the effects of combined application of Metarhizium rileyi Mr006 and chlorantraniliprole against Spodoptera frugiperda. Toxicity tests showed that, on the 7 th day after treatment with M. rileyi Mr006, LC₂₅, LC₅₀ and LC₉₀ against S. frugiperda were 8.97×10⁵ CFU/mL, 1.12×10⁷ CFU/mL and 1.37×10⁹ CFU/mL, respectively. For chlorantraniliprole treatment, the corresponding LC values on the 4 th day were 0.05 mg/L, 0.24 mg/L and 4.35 mg/L. Compatibility tests demonstrated that chlorantraniliprole at LC₂₅, LC₅₀ and LC₉₀ concentrations had no adverse effects on spore germination, mycelial growth or sporulation of M. rileyi Mr006. Regardless application sequence and interval time, all combined treatments significantly increased the corrected mortality of S. frugiperda, although the “fungus-first then insecticide” approach showed more pronounced synergistic effects than the reverse sequence. The highest corrected mortality reached 92.93% at 7 days post insecticide treatment following fungal treatment with a 36-hour interval. For mixing ratios, the 1:4 combination of M. rileyi Mr006 and chlorantraniliprole exhibited the most significant synergistic effect, achieving a 81.82% corrected mortality at 7 days post treatment. This study provides important theoretical basis and practical guidance for environmentally friendly control of S. frugiperda.

Key words: Metarhizium rileyi, chlorantraniliprole, Spodoptera frugiperda, toxicity, synergistic effect

中图分类号:

S476.2

毕思佳, 徐丽娜, 胡本进, 徐婷婷, 童友敏, 朱德涛, 祝子祥, 胡飞. 莱氏绿僵菌Mr006和氯虫苯甲酰胺协同应用对草地贪夜蛾的毒力[J]. 中国生物防治学报, 2026, 42(4): 818-827.

BI Sijia, XU Lina, HU Benjin, XU Tingting, TONG Youmin, ZHU Detao, ZHU Zixiang, HU Fei. Toxicity of Combined Application of Metarhizium rileyi Mr006 and Chlorantraniliprole against Spodoptera frugiperda[J]. Chinese Journal of Biological Control, 2026, 42(4): 818-827.

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